摘要
目的观察射干麻黄汤对哮喘小鼠模型气道炎症及血清白细胞介素6(IL-6)、白细胞介素10(IL-10)水平的影响。方法健康雄性SD小鼠60只,随机分为6组:空白对照组、哮喘模型组、地塞米松组、射干麻黄汤高、中、低剂量组,每组10只。采用卵清白蛋白(OVA)腹腔注射致敏及气道雾化激发构建小鼠哮喘模型,每组给予相对应的处置。末次干预24 h后,采集腹主动脉血、支气管肺泡灌洗液(BALF)、右肺下叶组织标本,分别运用ELISA法测定血清IL-6、IL-10的含量;光镜观察肺组织病理变化;HE染色对BALF中的细胞分类和计数。结果 1)射干麻黄汤高、中、低剂量组与哮喘模型组相比:IL-10水平升高(P<0.05)、IL-6水平下降(P<0.05)。与地塞米松组变化趋势相近。2)射干麻黄汤能明显减轻BALF中的嗜酸性粒细胞、中性粒细胞、淋巴细胞含量,减轻程度与地塞米松组相似。3)肺组织病理学变化哮喘模型组较空白对照组显著改变,治疗组较空白对照组呈不同程度改变、与哮喘模型组相比有一定差异。结论射干麻黄汤对哮喘小鼠的气道炎症具有一定的拮抗作用,其机制可能与降低血清IL-6、升高IL-10水平相关。
Objective： To observe the effect of Shegan Mahuang Decoction on asthma mouse model of airway in- flammation and serum IL-6 and IL-10 levels. Methods： 60 healthy male SD mice were randomly divided into 6 groups： control group,asthma model group,Shegan Mahuang Decoction high,medium and low dose group and dexamethasone group withl0 mice in each group. Mice models of asthma were established by intraperitoneal injection of OVA and airway atomization. Each group was given corresponding treatment. In 24 h after the last intervention, abdominal aortic blood,bronchoalveolar lavage fluid （BALF）,right lower lobe tissue specimens were collected;the serum levels of interleukin （IL-6） and interleukin （IL-10） were measured by ELISA method. The pathological changes of lung tissue were observed by light microscopy, and the HE staining was used to classify and count the cells in BALF. Results： 1 ）Compared with asthma model group,IL-lO level increased （P〈 0.05）, IL-6 decreased （P〈0.05） in Shegan Mahuang Decoction high,medium and low dose group. The trend of change was similar to that of dexamethasone group. 2）Shegan Mahuang Decoction could significantly reduce eosinophils, neutrophils,lymphocytes content in BALF,but not as effective as dexamethasone. 3）The pathological changes of lung tissue in model group was significantly more obvious than that in normal group ,and the treatment groups were significantly different than the normal group,and there was a certain difference compared with the model group. Conclusion ： Shegan Mahuang Decoction has certain antagonism on airway inflammation in asthmatic mice. Its mechanism may be related to the decrease of serum IL-6 and increase IL-10 level.
出处
《中国中医急症》
2017年第5期783-785,822,共4页
Journal of Emergency in Traditional Chinese Medicine
基金
黑龙江省自然科学基金项目(H201317)
