摘要
目的探讨胃癌组织和胃癌细胞株中长链非编码RNA结肠癌相关转录因子1（CCAT1）的表达情况及临床意义，并进一步探索CCAT1对胃癌细胞增殖能力的影响。方法通过实时荧光定量PCR（qRT—PCR）检测2013年1月至2015年5月期间于南京医科大学附属无锡第二医院普外科行胃癌根治术的62例胃癌患者的癌组织、对应癌旁正常组织以及胃癌细胞株中CCATl的mRNA表达水平，并通过统计学分析其临床意义。使用小干扰RNA（siRNA）分别转染胃癌细胞株MGC-803及SGC-7901，构建CCAT1低表达细胞系。在特定时间通过细胞计数试剂盒（CCK）-8法检测CCAT1对胃癌细胞增殖的影响。结果胃癌组织中CCAT1 mRNA表达水平较癌旁正常组织明显上调（3．394-2．37比1．28±0．74，P〈0．05）。胃癌细胞株MGC-803和SGC-7901中CCAT1 mRNA的相对表达水平均高于胃黏膜永生细胞系GES-1（3．07±0．69、2．23±O．32比1．01±0．12，均P〈0．05）。不同TNM分期、肿瘤浸润深度和淋巴结转移的患者胃癌组织中CCAT1表达水平不同，差异均有统计学意义（χ^2=5．199、5．395、9．239，均P〈0．05）。CCK-8细胞增殖实验结果显示，分别在MGC-803及SGC-7901细胞中抑制CCAT1的表达后，其细胞增殖能力受到明显抑制，差异均有统计学意义（均P〈0．05）。结论胃癌中CCATl的表达水平上调并且与胃癌患者的病情进展密切相关，下调CCAT1的表达水平可以抑制胃癌细胞的增殖。CCAT1有可能成为胃癌早期诊断以及治疗的新靶点。
Objective To investigate the expression and clinical significance of long non-coding RNA colon cancer associated transcript-1 （ CCAT1 ） in gastric cancer （ GC）, and to further explore the effect of CCAT1 on cell proliferation of GC. Methods The mRNA expressions of CCAT1 in GC tissues and matched adjacent normal tissues from 62 patients who received resection for gastric carcinoma between January 2013 and May 2015 in Nanjing Medical University Affiliated Wuxi Second Hospital and expressions in GC cell lines were assessed by quantitative real-time PCR （qRT-PCR）. The clinical significance of CCAT1 expression was then analyzed. The expressions of CCAT1 in MGC-803 and SGC-7901 cells were inhibited by small interfering RNA （ siRNA ） transfection. The effect of CCAT1 on cell proliferation was studied by cell counting kit （CCK）-8 assay. Results The expressions of CCAT1 mRNA in GC tissues were significantly higher than in the normal tissues （ 3.39 ± 2. 37 vs 1.28 ± 0. 74, P 〈 0. 05 ）. Compared with immortalized human gastric epithelial cell line （ GES-1 ）, the expressions of CCAT1 mRNA were significantly higher in GC cell lines MGC-803 and SGC-7901 （3.07 ± 0. 69, 2. 23 ±0. 32 vs 1.01 ± 0. 12, both P 〈 0. 05 ）. Besides, the expression of CCAT1 varied significantly among patients with different TNM stage, depth of invasion, and lymph node metastasis （ χ^2 = 5. 199, 5. 395, 9. 239, all P 〈 0. 05）. The results of CCK-8 assay showed that down-regnlation of CCAT1 in MGC-803 and SGC-7901 cells significantly inhibited the cell proliferation （ both P 〈 0. 05 ）. Conclusions CCAT1 is up-regulated in GC and may be significantly correlated with the progression of GC. Decreased expression of CCAT1 can suppress the proliferation of GC cells. CCAT1 might be used as a novel target for GC early diagnosis and treatment.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2017年第18期1411-1414,共4页
National Medical Journal of China
