摘要
目的建立固相萃取-手性气相色谱测定醒脑静注射液中右旋龙脑和麝香酮含量的方法。方法醒脑静注射液直接经C18固相萃取柱前处理,用无水乙醇洗脱,洗脱液以手性气相色谱柱测定右旋龙脑和麝香酮的含量。毛细管色谱柱为CYCLOSIL-B(30m×0.25mm×0.25μm),进样口为200℃,FID检测器为220℃,采用程序升温118℃保持2min,以50℃/min升至220℃保持10min。结果右旋龙脑的平均回收率为100.4%,RSD=0.9%(n=6),线性范围0.048mg·mL^(-1)~0.57mg·mL^(-1),r=0.9999;麝香酮的平均回收率为96.5%,RSD=0.9%(n=6),线性范围2.3μg·mL^(-1)~23.2μg·mL^(-1),r=0.9999。结论该方法前处理非常简便,结果准确可靠,可用于醒脑静注射液的质量控制。
OBJECTIVE To establish a methods to simultaneously determine the contents of D-bomeol and muscone in Xingnaojing Injection by Solid-Phase Extraction （SPE） and Chiral Capillary Gas Chromatogra- phy. METHODS After pretreatment by C lS SPE column, Chiral Capillary Column of CYCLOSIL-B （30m ×0. 25mm×0. 25μm） was used for D-borneol and muscone in Xingnaojing Injection. Injector temperature was 200℃ and FID detector temperature was 220℃. The column temperature was programmed with initial temperature at 118℃ held for two minutes,then raised to 220-C at a rate of 50℃/min which was kept for ten minutes. RESULTS D-borneol and muscone showed good linear relationships within the range of 0. 048mg·mL^-1 - 0. 57mg·mL^-1 （r = 0. 9999 ） and 2.3μg·mL^-1-23.2μg·mL^-1 （r = 0. 9999 ） , respectively. The average recoveries of D-bomeol and museone were 100. 4% and 96.5% with RSDs of 0. 9% （ n = 6）. CONCLUSION The developed method is simple, precise and reliable, which could be applied for the quality control of Xingnaojing Injection.
出处
《海峡药学》
2017年第4期68-70,共3页
Strait Pharmaceutical Journal
