摘要
目的:探讨甲状腺癌超声弹性应变率比值与病灶组织中癌细胞恶性生物学特征的相关性。方法:选取在本院接受手术治疗的甲状腺癌患者90例作为观察组,另取同期在本院接受手术、病理明确为甲状腺腺瘤的患者50例作为对照组。根据超声弹性应变率比值中位数将观察组进一步分为高弹性应变率比值组、低弹性应变率比值组各45例。采用RT-PCR法测定各组患者病灶组织中的增殖基因、侵袭基因、自噬基因mRNA表达量。结果:观察组的平均弹性应变率比值显著低于对照组(P<0.05)。低弹性应变率比值组、高弹性应变率比值组病灶组织中增殖基因FOXA1mRNA表达量高于对照组,PP4R1、Smac mRNA表达量低于对照组,弹性应变率比值降低,FOXA1 mRNA表达量增加,PP4R1、Smac mRNA表达量降低(P<0.05);低弹性应变率比值组、高弹性应变率比值组病灶组织中侵袭基因BCORL1、SATB1、Twist1、CXCR4 mRNA表达量高于对照组,弹性应变率比值降低,BCORL1、SATB1、Twist1、CXCR4mRNA表达量增加(P<0.05);低弹性应变率比值组、高弹性应变率比值组病灶组织中自噬基因ARHI、Beclin1、LC3、PTEN mRNA表达量低于对照组,弹性应变率比值降低,ARHI、Beclin1、LC3、PTEN mRNA表达量降低(P<0.05)。结论:甲状腺癌超声弹性应变率比值与肿瘤细胞增殖、侵袭活性呈负相关,与自噬活性呈正相关。
Objective： To study the correlation between the ultrasonic elastic strain ratio of thyroid cancer and the malignant biological characteristics of cancer cells in the lesions. Methods： A total of 90 patients with thyroid cancer who accepted surgical treatment in our hospital between March 2015 and September 2016 were selected as the observation group, and 50 patients who received surgery in our hospital during the same period and were with clear pathological diagnosis of thyroid adenoma were selected as the control group. According to the median of ultrasonic elastic strain ratio, the observing group were ：further divided into high elastic strain ratio group and low elastic strain ratio group （n =45）. RT-PCR was used to determine the mRNA expression of proliferation genes, invasion genes and autophagy genes in lesions of all groups of patients. Results： The mean elastic strain ratio of observation group was significantly lower than that of control group （P〈0.05）. Proliferation gene FOXA1 mRNA expression in lesions of low elastic strain ratio group and high elastic strain ratio group were higher than those of control group while PP4R1 and Smac mRNA expression were lower than those of control group, and as the elastic strain ratio decreased, the FOXA1 mRNA expression increased while PP4R1 and Smac mRNA expression decreased （P〈0.05） ; invasion genes BCORL1, SATB1, Twistl and CXCR4 mRNA expression in lesions of low elastic strain ratio group and high elastic strain ratio group were higher than those of control group, and as the elastic strain ratio decreased, the BCORL1, SATB1, Twist1 and CXCR4 mRNA expression increased （P〈0.05） ; autophagy genes ARHI, Beclinl, LC3 and PTEN mRNA expres sion in lesions of low elastic strain ratio group and high elastic strain ratio group were lower than those of control group, and as the elastic strain ratio decreased, the ARHI, Beclinl, LC3 and PTEN mRNA expression decreased （P〈0.05）. Conclusions： The ultrasonic elastic strain ratio of thyroid cancer is negatively correlated with tumor cell proliferation and invasion activity, and positively correlated with autophagy activity.
出处
《海南医学院学报》
CAS
2017年第8期1138-1141,1145,共5页
Journal of Hainan Medical University
基金
四川省教育厅项目(14ZB0311)~~
关键词
甲状腺癌
超声弹性应变率比值
增殖基因
侵袭基因
自噬基因
Thyroid cancer
Ultrasonic elastic strain ratio
Proliferation gene
Invasion gene
Autophagy gene
