摘要
采用PCR-DGGE方法研究活性污泥和土壤中微生物种群结构的变化.提取样品总DNA作为模板,采用细菌通用引物GC-338F和518R进行PCR扩增.最后对DGGE图谱中优势条带进行回收、测序.结果显示,变形菌门和拟杆菌门是构成土壤和污泥微生物群落的主要种群,且变形菌门所占比例最大,在各样品中均占40%以上,是该研究样品中的优势细菌类群;土壤的细菌群落中检测到放线菌门,而活性污泥中未检出.
The purpose of this study was to explore the changes of microbial community structure in activated sludge and soil by PCR-DGGE method. Total DNA was extracted as template, GC-338F and 518R were used as bacterial universal primers to amplify the extracted DNA by PCR, and the dominant bands were recovered and sequenced in DGGE map. The results suggested that Proteobacteria and Bacteroidetes were the main species composition of soil and sludge microbial communities, as the dominant bacteria, Proteobacteria accounted for more than 40% in each sample of this study; Actinobacteria were detected in the soil bacterial community, but not in the activated sludge.
出处
《南通大学学报（自然科学版）》
CAS
2017年第1期35-38,共4页
Journal of Nantong University(Natural Science Edition)
基金
国家自然科学基金项目(21177067)
