摘要
【目的】为了分析和鉴定鱼腥草挥发油的化学组成,测定鱼腥草挥发油和乙酸乙酯层次生代谢产物对7种供试细菌的抑制活性。【方法】本文采用水蒸汽蒸馏法和甲醇冷浸提取法分别提取鱼腥草挥发油和乙酸乙酯层次生代谢产物,通过GC-MS对提取得到的挥发油进行化学成分分析,采用抑菌圈法和TIC-MTT-生物自显影法分别测定挥发油和乙酸乙酯层次生代谢产物对供试细菌的抑制活性。【结果】鱼腥草挥发油的得率为0.09%,从鱼腥草挥发油中共鉴定出49个组分,占总相对含量的93.35%,主要成分是β-蒎烯(23.65%)、2-十三烷酮(12.38%)、甲基正壬酮(8.55%)、乙酸冰片酯(4.08%)、月桂酸(3.77%)、1-十三烯(3.51%)、氧化石竹烯(2.97%)和香附烯(2.59%)等。鱼腥草挥发油对7种供试细菌均表现出一定的抑菌作用,其中对黄瓜角斑病菌的抑制活性最强,抑菌圈直径为(20.0±2.0)mm,而对溶血葡萄球菌的抑制活性最弱,抑菌圈直径仅为(8.7±0.6)mm;对桉树青枯病菌和黄瓜角斑病菌的抑制活性要强于硫酸链霉素。鱼腥草乙酸乙酯层次生代谢产物未表现出任何抗菌活性。【结论】鱼腥草中含有丰富的挥发性成分,且抗菌活性物质主要分布于挥发油中;鱼腥草挥发油对桉树青枯病菌和黄瓜角斑病菌表现出较好的抑制活性,对革兰氏阴性菌的抑制活性要强于革兰氏阳性菌。
[ Objective ] To analyze and identify the chemical composition of volatile oil extracted from Houttuynia cordata, the inhibition activi- ties of volatile oil and ethyl acetate extracts against 7 different bacteria were determined. [ Method] The volatile oil from Houttuynia cordata was extracted by hydro-distillation, and the ethyl acetate extracts was extracted by methanol extraction at room temperature. Gas chromatogra- phy-mass spectrometry （GC-MS） was used to analyze the chemical compositions of the essential oil, and inhibition zone method and TLC- MTT-bioautography assay were used to detect the inhibitory activities of the volatile oil and ethyl acetate extracts against bacteria, respective- ly. [ Result]The essential oil from fresh weight basis of Houttuynia cordata was extracted with the yield of 0.09 %, and forty-nine compo- nents from the volatile oil were identified, which accounted for 93.35 % of total contents, of which fl-Pinene （23.65 % ） , 2-Tridecanone （ 12.38 % ）, 2-Undeeanone （8.55 % ）, Bornyl acetate （4.08 % ）, Dodeeanoic acid （3.77 % ）, 1-Tridecene （3.51% ）, Caryophynene oxide （2.97 % ） and a-Cyperene （2.59 % ） were the major compounds. The volatile oil had certain antibacterial activities on 7 test strains, which showed the strongest inhibitory activity against Pseudomonas lachrymans and the weakest on Staphylococcus haernolyticus, and the inhi-bition zone diameters were （20.0 ± 2.0） and （8.7 ± 0.6） mm, respectively. The antibacterial activities of the volatile oil against Ralstoniasolanacearum and Pseudomonas lachrymans were better than that of streptomycin sulfate. The ethyl acetate extracts from Houttuynia cordata showed no antibacterial activi- ty on test bacteria. [ Conclusion ] Houttuynia cordata was rich in volatile components, and the antibacterial active substances were mainly distributed in the volatile oil. The volatile oil showed better inhibitory activities against Ralstoniasolanacearum and Pseudomonas lachrymal. The inhibitory activity of volatile oil on gram- negative bacteria was stronger than gram-positive bacteria.
出处
《西南农业学报》
CSCD
北大核心
2017年第5期1041-1047,共7页
Southwest China Journal of Agricultural Sciences
基金
广东省普通高校青年创新人才项目(2014KQNCX034)
广东省林业科技创新项目(2015KJCH043)
广州市珠江科技新星专项(201506010029)
关键词
鱼腥草
次生代谢产物
抗细菌活性
Houttuynia cordata
Secondary metabolites
Antibacterial activities
