摘要
目的探讨血浆中miRNA-126和miRNA-28-3p的表达与糖尿病(DM)的关系,并进行相关机制探讨。方法随机选取恩施土家族苗族自治州中心医院DM患者80例作为DM组,选取同期治疗的非DM患者80例作为对照组,采用qRT-PCR检测其血浆中循环miRNA-126和miRNA-28-3p的表达水平,并对其靶基因、基本生物学功能和相关lncRNA和circRNA进行生物信息学预测。结果 DM患者血浆中miRNA-126的表达水平(0.115 0±0.014 4)较对照组患者(0.001 9±0.000 6)明显升高,差异具有极显著性统计学意义(P<0.01);DM患者血浆中miRNA-28-3p的表达水平(0.138 6±0.017 24)较对照组患者(0.000 6±0.000 05)明显升高,差异具有极显著性统计学意义(P<0.01);miRNA-126与miRNA-28-3p的Pearson相关系数为0.433 5(P<0.01);2组患者miRNA-126、miRNA-28-3p ROC曲线下面积差异均有极显著性统计学意义(P<0.01);生物信息学分析发现miRNA-126、miRNA-28-3p可能参与调控了胰岛素信号通路、胰岛素受体信号通路、胰岛素/胰岛素生长因子信号通路、丝裂原活化蛋白激酶(MAPK)信号通路和血管生成等信号通路,并可能与多种lncRNA和circRNA都有关。结论血浆中循环miRNA-126与miRNA-28-3p可能通过调控胰岛素和胰岛素生长因子相关信号通路导致DM的发生,可以作为DM新型临床诊断生物标志物,并可能与多种lncRNA和circRNA有关。
Objective To explore the mechanism of plasma circulating miRNA-126 and miRNA-28-3p in diabetes mellitus （DM） pa- tients, and to identify the related bioinformatics analysis. Methods Randomly selected 80 DM patients as the observation group and 80 non- DM patients as the control group. The plasma circulating miRNA-126 and miRNA-28-3p were analyzed by qRT-PCR,and its target genes, bio- logical information,related lneRNA and circRNA were predicted. Results The circulating miRNA-126 （0.115 0 ± 0. 014 4 vs. 0. 0019 ± 0.000 6） and miRNA-28-3p（0. 1386 ± 0. 01724 vs. 0. 000 6 ± 0. 000 05 ） levels in the observation group were significantly higher than those in the control group, and the differences were statistically significant （P 〈 0.01 ）. Pearson correlation coefficient of miRNA-126 and miRNA- 28-3p was 0. 433 5（P 〈0.01 ）. ROC curve analysis of miRNA-126 and miRNA-28-3p showed that the differences of the area under curve were statistically significant between the two groups （P 〈 0.01 ）. Bioinformatics prediction showed that miRNA-126 and miRNA-28-3p may be involved in regulation of the insulin signaling pathway, insulin receptor signaling pathway,insulin/insulin growth factor signaling pathway, mi- togen-activated protein kinase （MAPK） signaling pathway and angiogenesis. And it may be associated with a variety of lucRNA and eir- cRNA. Conclusion Circulating miRNA-126 and miRNA-28-3p can be a novel biomarker of DM as it may participate in the mechanism of DM by regulating insulin and insulin growth factor related signaling pathways and be associated with some related lncRNA and cireRNA.
作者
邓仁生
朱小琴
刘长召
王玲
陈文江
DENG Ren-sheng ZHU Xiao-qin LIU Chang-zhao WANG Ling CHEN Wen-jiang(Department of Endocrinology, Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi Hubei 445000, China Department of Cardiology, Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi Hubei 445000, China Department of Cardiovascular, Affiliated Hospital of Guang- dong Medical University, Zhanjiang Guangdong 524001, China)
出处
《局解手术学杂志》
2017年第6期400-405,共6页
Journal of Regional Anatomy and Operative Surgery
