摘要
目的:建立同时测定减肥降脂保健品中儿茶素类活性成分含量的方法。方法:采用高效液相色谱法,以表没食子基儿茶素没食子酸酯(EGCG)为内标,计算其与没食子儿茶素(EGC)、儿茶素(C)、表儿茶素(EC)、没食子基儿茶素没食子酸酯(GCG)和没食子酰表儿茶素(ECG)的相对校正因子(RCF),通过RCF计算样品中EGC、C、EC、GCG、ECG的含量,以外标法测定的EGC、C、EC、GCG和ECG含量作实测值;采用向量夹角余弦法评估实测值与计算值之间的相似性。结果:EGC、C、EC、EGCG、GCG、ECG检测质量浓度线性范围分别为0.006 5~0.130 5 mg/m L(r=0.999 8)、0.000 5~0.010 7 mg/m L(r=0.999 7)、0.002 0~0.040 0mg/m L(r=0.999 9)、0.015 3~0.305 3 mg/m L(r=0.999 8)、0.000 8~0.015 5 mg/m L(r=0.999 8)、0.004 0~0.079 2 mg/m L(r=0.999 9);精密度、稳定性、重复性试验的RSD<2.0%;加样回收率分别为95.07%~100.35%(RSD=1.94%,n=6)、95.24%~101.87%(RSD=2.79%,n=6)、96.08%~103.86%(RSD=3.01%,n=6)、97.51%~101.06%(RSD=1.45%,n=6)、96.01%~101.66%(RSD=2.27%,n=6)、96.20%~102.89%(RSD=2.71%,n=6)。实测值与计算值差异无统计学意义(P>0.05)。结论:该方法操作简便,精密度、稳定性、重复性好,可用于减肥降脂保健品中儿茶素类活性成分的含量测定。
OBJECTIVE： To establish the method for contents determination of catechins active components in lipid-lowering slimming health products. METHODS： HPLC method was adopted. Using epigallocatechin gallate （EGCG） as a reference, relative correction factor （RCF） of EGCG to gallocatechin （EGC）, catechin （C）, epicatechin （EC）, gallocatechin gallate （GCG） and galloylepicatechin（ECG）were calculated. The contents of EGC, C, EC, GCG and ECG in 5 batches of samples were calculated through RCF. The contents of EGC, C, EC, GCG and ECG determined by external standard method were used as measured value. The similarity of the value determined by external standard method with the value calculated by quantitative analysis of multi-components via single marker method （QAMS） was evaluated with vector included angle cosine method. RESULTS： The linear ranges of EGCG,EGC,C,EC,GCG and ECG were 0.006 5-0.130 5 mg/mL（r=0.999 8）~0.000 5-0.010 7 mg/mL（r=0.999 7）~0.002 0- 0.040 0 mg/mL（r=0.999 9）~0.015 3-0.305 3 mg/mL（r=0.999 8）,0.000 8-0.015 5 mg/mL （r= 0.999 8）~0.004 0-0.079 2 mg/mL （r=0.999 9）; RSDs of precision, stability and reproducibility tests were all lower than 2.0%.The recoveries were 95.07%-100.35% （RSD= 1.94%, n=6） ,95.24%-101.87% （RSD=2.79%, n=6） ~ 96.08 %-103.86% （RSD= 3.01%, n=6） ,97.51%-101.06% （RSD= 1.45% ,n=6） ~96.01%-101.66% （RSD=2.27% ,n=6） ~96.20%-102.89% （RSD=2.71% ,n=6）, respectively. There was no significant difference between measured value and calculated value. CONCLUSIONS： The method is simple, precise, stable and reproducible, and can be used for contents determination of catechins active components in lipid-lowering slimming health products.
出处
《中国药房》
CAS
北大核心
2017年第18期2529-2532,共4页
China Pharmacy
基金
国家自然科学基金资助项目(No.81503361)
四川省教育厅科研计划项目(No.16ZA0299)
关键词
减肥
保健品
儿茶素
一测多评法
高效液相色谱法
Slimming
Health products
Catechins
Quantitative analysis of multi-components via single marker method
HPLC
