七氟醚预处理对单肺通气患者术侧肺组织自噬的影响被引量：7

Effect of sevoflurane preconditioning on autophagy in operated lung tissues of patients undergoing one-lung ventilation

原文传递

导出

摘要目的评价七氟醚预处理对单肺通气患者术侧肺组织自噬的影响。方法择期全麻下行胸腔镜下肺癌根治术患者40例，ASA分级Ⅰ-Ⅲ级，性别不限，年龄30～80岁，体重45～84 kg，采用随机数字表法分为2组（n＝20）：对照组（C组）和七氟醚预处理组（SP组）。SP组于单肺通气前吸入七氟醚，呼气末浓度为2.0%，吸入30 min后洗出。取距离肿块边缘〉2 cm的正常肺组织，采用Western blot法检测微管相关蛋白1轻链3Ⅱ的表达水平。结果与C组比较，SP组肺组织微管相关蛋白1轻链3Ⅱ表达上调（ P〈0.05）。结论七氟醚预处理可增强单肺通气患者术侧肺组织自噬，该作用可能与其肺保护效应有关。 Objective To evaluate the effect of sevoflurane preconditioning on autophagy in the operated lung tissues of the patients undergoing one-lung ventilation.Methods Forty patients of both sexes, with American Society of Anesthesiologists physical status Ⅰ-Ⅲ, aged 30-80 yr, weighing 45-84 kg, scheduled for elective radical operation for lung cancer performed via video-assisted thoracoscope under general anesthesia, were divided into control group（C group）and sevoflurane preconditioning group（SP group）, with 20 patients in each group.In SP group, sevoflurane was inhaled for 30 min with the final concentration of 2.0%, followed by washout.The normal lung tissues（the distance from the edge of the lump〉2 cm）around the lump were obtained for detection of the expression of microtubule-associated protein 1 light chain 3 Ⅱ by Western blot.Results Compared with C group, the expression of microtubule-associated protein 1 light chain 3 Ⅱ in lung tissues was significantly up-regulated in SP group（P〈0.05）.Conclusion Sevoflurane preconditioning can enhance autophagy in the operated lung tissues of the patients undergoing one-lung ventilation, which may be related to the lung protective effect.

作者韩杨杨朱浩张凯王妍黄长顺方向明

机构地区浙江大学医学院附属第一医院麻醉科宁波市第一医院麻醉科

出处《中华麻醉学杂志》 CSCD 北大核心 2017年第4期417-419,共3页 Chinese Journal of Anesthesiology

关键词麻醉药吸入缺血预处理自噬呼吸人工呼吸窘迫综合征成人 Anesthetics, inhalation Ischemic preconditioning Autophagy Respiration, artificial Respiratory distress syndrome, adult

分类号 R614 [医药卫生—麻醉学]

引文网络
相关文献

参考文献1

1陶洪霞,沈启英,李元海.七氟醚预处理对单肺通气时肺顺应性和氧合指数的影响[J].安徽医科大学学报,2016,51(5):682-685. 被引量：22

二级参考文献12

1Karzai W, Schwarzkopf K. Hypoxemia during one - lung ventila- tion : prediction, prevention, and treatment [ J ]. Anesthesiology, 2009, 110(6) : 1402 -11.
2Xu W Y, Wang N, Xu H T, et al. Effects of sevoflurane and propofol on right ventricular function and pulmonary circulation in patients undergone esophagectomy [ J ]. Int J Clin Exp Pathol, 2013, 7(1) : 272 -9.
3Jin Y, Zhao X, Li H, et al. Effects of sevoflurane and propofol on the inflammatory response and pulmonary function of perioperative patients with one-lung ventilation [ J]. Exp Ther Med,2013, 6 (3) : 781 -5.
4Schilling T, Kozian A, Kretzschmar M, et al. Effects of propofol and desflurane anaesthesia on the alveolar inflammatory response to one - lung ventilation [ J ]. Br J Anaesth, 2007, 7 (3) : 368 - 75.
5Shen Q Y, Fang L, Wu H M, et al. Repeated inhalation of sevoflurane inhibits airway inflammation in an OVA -induced mouse model of allergic airway inflammation [ J ]. Respirology, 2015, 20(2) : 258 -63.
6Voigtsberger S, Lachmann R A, Leutert A C, et al. Sevoflurane ameliorates gas exchange and attenuates lung damage in experi- mental lipopolysaceharide - induced lung injury [ J ]. Anesthesiolo- gy , 2009, 111(6) : 1238 -48.
7Schwarzkopf K, Schreiber T, Preussler N P, et al. Lung perfu- sion, shunt fraction, and oxygenation during one - lung ventilation in pigs : the effects of desflurane, isoflurane, and propofol [ J ]. J Cardiothorac Vase Anesth, 2003, 17(1 ): 73-5.
8Karci A, Duru S, Hepaguslar H, et al. Comparison of the effect ofsevoflurane and propofol on oxygenation during gradual transition to one - lung ventilation [J]. Braz .1 Anesthesiol, 2014, 64 ( 2 ) : 79 -83.
9Schwarzkopf K, Hueter L, Schreiber T, et al. Oxygenation during one - lung ventilation with propofol or sevoflurane [ J ]. Middle East J Anaesthesiol, 2009, 20 (3) : 397 - 400.
10Myers C F, Fontao F, Jrnosi T Z, et al. Sevoflurane and desflu- rane protect cholinergic - induced bronchoconstriction of hyperre- active airways in rabbits [ J]. Can J Anaesth, 2011, 58 ( 11 ) : 1007 - 15.