摘要
目的:分析越南和国产土茯苓化学成分的差异,对差异性化合物进行辨识,并对样品进行产地鉴别。方法:收集越南土茯苓7份,国产土茯苓28份。建立越南和国产土茯苓化学成分色谱指纹图谱,根据相似度评价结果比较两者的差异程度,采用HPLC-MS对指纹图谱的主要色谱峰进行归属,共鉴定21个峰。采用聚类分析对样品进行产地鉴别;结合正交信号校正偏最小二乘判别分析(OPLS-DA)进行差异性化合物的筛选,选取位于S-plot图两端并且VIP>1的点作为区分越南与国产土茯苓的差异性化合物。结果:越南和国产土茯苓主要化学成分的含量存在差异性,聚类分析可将35批土茯苓药材分为越南和国产2组,两者的差异性化合物分别为落新妇苷、异落新妇苷、新落新妇苷、黄杞苷,越南土茯苓中差异性化合物的含量显著大于国产土茯苓。结论:采用OPLS-DA分析样品的指纹图谱,既可从整体角度分析越南和国内土茯苓化学成分的异同,又可对两者差异性化合物进行辨识,为越南土茯苓入药的可能性及其质量评价提供实验数据。
Objective： To analyze the chemical differences of Smilacis Glabrae Rhizome from Vietnam and China, identify discrimination markers, and establish the method of identifying the origins of samples. Method： Samples were collected from Vietnam （7 batches） and China （28 batches）. The chromatography fingerprints of the above samples were established, and the differences between the two kinds of samples were compared according to the similarity evaluation results. At the same time, the main chromatographic peaks were identified by HPLC-MS, and 21 peaks were identified. Cluster analysis was used to identify the origin of the samples. Orthogonal partial least squares discriminant analysis （OPLS-DA） was used to determine the discrimination markers between samples collected from Vietnam and China, and the points with VIP 〉 1 at theends of S-plot were selected as potential markers. Result： Differences in main chemical compounds were found between the samples from Vietnam and China. Thirty-five samples were divided into Vietnam group and China group. Astilbin, isoastilbin, neoastilbin and engeletin were determined to be the discrimination markers, and their contents in samples of Vietnam were obviously higher than those in samples of China. Conclusion： Fingerprint coupled with OPLS-DA can be used to analyze the chemical similarity and differences between samples from Vietnam and China. The results provided data not only for the quality evaluation of samples from Vietnam, but also for the answer on whether samples from Vietnam can be used as medicine.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2017年第14期62-67,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
中医药行业科研专项(201407003)
