摘要
目的研究阿纳其(罗马除虫菊Anacyclus pyrethrum)根提取物对慢性抑郁模型和药物诱发抑郁模型的影响。方法采用慢性温和不可预知应激(CUMS)刺激的方式制备外源性大鼠抑郁模型,通过糖水偏好度、旷场实验、强迫游泳实验、悬尾实验考察大鼠行为学变化;利用Elisa法检测大鼠血清皮质酮的量与海马中5-羟色胺(5-HT)和多巴胺(DA)的量。采用药物诱导方式制备内源性小鼠抑郁模型,分别采用利血平拮抗实验、5-羟色氨酸(5-HTP)诱导甩头实验和育亨宾毒性实验建立内源性小鼠抑郁模型,通过观察小鼠眼睑下垂动物数、运动不能动物数、甩头次数和死亡率,研究阿纳其根提取物抗抑郁作用。结果慢性抑郁模型中,阿纳其根提取物可明显提高CUMS抑郁模型大鼠糖水偏好度、旷场实验中水平穿格次数和直立次数,显著降低强迫游泳和悬尾实验中大鼠的不动时间,同时抑制大鼠血清中皮质酮水平,促进大鼠海马中5-HT和DA水平的升高。药物诱发抑郁模型中,阿纳其根提取物能够减少利血平诱导小鼠的眼睑下垂动物数和运动不能动物数,显著提高5-HTP诱导小鼠的甩头次数,但对育亨宾诱导致死小鼠数量没有影响。结论阿纳其根提取物对慢性抑郁大鼠和药物诱导的抑郁小鼠有一定的抗抑郁作用,其作用机制可能与降低皮质酮和促进5-HT神经功能有关。
Objective To study the antidepressant effect of Anacycli Pyrethri Radix extract（APRE） in chronic unpredictable mild stress（CUMS） model and drug-induced depression model. Methods The chronic unpredictable stimulus was used to establish exogenous chronic stress depression model in rats. The sucrose preference, open-field test, forced swimming test and tail suspension test were used to observe the behavioral changes of rats. Serum levels of corticosterone and 5-HT and DA of hippocampus were analyzed using ELISA. Reserpine reversal test, 5-HTP induced head-twitches test and yohimbine induced lethality were used to establish endogenous mouse depression model; Blepharoptosis, akinesia, head-twitch cunts and mortality were observed to study he antidepressant effect of APRE. Results In the chronic unpredictable stress model, APRE could increase the sucrose intake, crossing numbers and rearing numbers obviously, the immobility time was reduced, the level of the serum corticosterone was reduced significantly, the 5-HT and DA of hippocampus were increased. In the drug-induced depression model, blepharoptosis and akinesia incuced by reserpine were inhibited, head-twitches induced by 5-HTP was significantly increased, but there was no effect on mortality induced by yohimbine. Conclusion APRE has obvious antidepressant effect possibly by decreasing corticosterone level and promoting 5-HT nerve function.
出处
《中草药》
CAS
CSCD
北大核心
2017年第12期2492-2497,共6页
Chinese Traditional and Herbal Drugs
基金
新疆维吾尔自治区科技人才培养项目(qn2015yx042)
新疆维吾尔自治区重点研发计划项目(2016B03038-3-3)
