摘要
为了比较火龙果与昙花的成分差异,以火龙果与昙花的茎为试验材料,采用红外光谱、二阶导数光谱和二维相关同步光谱技术对其成分进行了分析。结果表明:红外光谱图显示火龙果茎有5个明显的特征峰,昙花茎有4个;其中两者相同的吸收峰对应的官能团为C—O—C(脂肪烃)、C—O—C、N—H、O—H(二聚体),不同的吸收峰为1 041 cm^(-1)(火龙果)、1 077 cm^(-1)(火龙果)、1 033 cm^(-1)(昙花);667~1 866 cm^(-1)区间的二阶导数红外光谱图显示,火龙果茎有14个吸收峰,昙花茎有16个,955 cm^(-1)为火龙果茎特有的吸收峰,对应的官能团为酸酐C—O;1 520 cm^(-1)为昙花茎特有的吸收峰,对应的官能团为胺N—H;890~1 800 cm^(-1)区间的二维相关同步红外光谱图显示,火龙果茎和昙花茎的交叉峰相同,其中1 321 cm^(-1)与1 630 cm^(-1)形成了较强正交叉峰,1 046 cm^(-1)与1 634 cm^(-1)、1 049 cm^(-1)与1 329 cm^(-1)形成了较弱的正交叉峰,无负交叉峰;二维相关红外光谱图显示的自动峰位置与红外光谱的一致。
In order to explore the component difference between Hylocereus undlatus and Epiphyllum oxypetalum, fourier transform infrared spectroscopy （FTIR） , second derivative infrared spectroscopy and two-dimensional correlation infrared spectroscopy （2D-IR） were analysed with their stems. The infrared spectrogram showed that there were five absorption peaks in stem of H undlatus, and four in E. oxypetalum; The similar peaks of both represent the same functional groups which were C-O-C （aliphatic hydrocarbon）, C--O--C, N--H, O--H （dimer） . But the differences were 1 041 cml H. undlatus, 1 077 cm-1H. undlatus, 1 033cm1 E. oxypetalum. The fourteen intensities absorptionpeaks was shown in stem ofH. undlatus and sixteenin E. oxypetalum according to the second derivative infrared spectroscopy in the range of 667-1 866 cm1. An obvious characteristic peak was 955 cml in H. undlatus corresponded to the functional group of acid anhydride C--O, and 1 520 cm1 in E. oxypetalum corresponded to the functional group of amine N--H. There were same cross peaks between H. undlatus and E. oxypetalum, on map of 2D-IR spectroscopy in the range of 890-1 800 cm1 especially a strong positive cross peaks in the wave number of 1 321 cm^-1and 1 630 cm^-1, weaker positive cross peaks in the wave number of 1 046 cm^-1 ＆l 634 cm^-1, 1 049 cm^-l ＆ 1 329 cm1, without negative cross peak.The positions of automatic spectrum peak was same with the fourier transform infrared spectroscopy （FTIR） on two-dimensional correlation infrared spectroscopy （2D-IR） .
出处
《天津农学院学报》
CAS
2017年第2期30-33,共4页
Journal of Tianjin Agricultural University
基金
天津市农业科技成果转化与推广项目"彩叶树优良品种繁殖
栽培技术示范与推广"(201502100)
天津市科技成果转化及产业化推进计划项目"优质彩叶北美海棠新品种种苗的规模化繁育"(14ZXNZNC0040)
关键词
火龙果
昙花
红外光谱
二阶导数
二维相关
Hylocereus undlatus
Epiphyllum oxypetalum
infrared spectrum
second derivative spectrometry
two-dimensionalcorrelation spectroscopy
