摘要
为评估乙基多杀菌素在香蕉上的安全性使用,本研究采用超高效液相色谱-串联质谱(UPLC-MS/MS)法,检测香蕉植株和土壤中乙基多杀菌素的残留及降解动态。结果表明,乙基多杀菌素在香蕉果实、花瓣和土壤中的检出限(LOD)均为5μg/kg,定量限(LOQ)均为17μg/kg。当乙基多杀菌素在果实、花瓣和土壤中的添加浓度在0.01~0.20 mg/kg时,添加回收率分别为89.6%~95.2%、76.5%~89.8%和75.8%~86.5%,相对标准偏差分别为5.03%~7.10%、5.16%~7.24%和2.26%~5.83%。乙基多杀菌素在香蕉果实、花瓣和土壤中的半衰期分别为5.6、4.5和6.5 d,属于易消解型农药。UPLC-MS/MS法具有灵敏度高、速度快等特点,可利用此法检测香蕉中乙基多杀菌素残留及消解动态。
In order to evaluate the safety of spinetoram on bana na, the residue and degradation dynamics of spinetoram in banana were detected by ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS）. The results showed that the limits of detection（LOD） of spinetoram in banana fruit, petal and soil were both 5 μg/kg. And the limits of quantification（ LOQ） were both validated calculated as 17 μg/kg. At the spiked levels of 0.01-0.20 mg/kg, the average recoveries of acetochlor in fruit, petal and soil were 89.6%-95.2%,76.5%-89.8% and 75.8%-86.5%, and the relative standard deviations was 5.03%-7.10%, 5.16%-7.24% and2.26%-5.83%, respectively. The half-lives of acetochlor in fruit, petal and soil was 5.6 d, 4.5 d and 6.5 d. Taken together, the findings reveal that spinetoram belongs to the easily degradable pesticide type. The present study suggests that UPLC-MS/MS could be applied to detect and analyze spinetoram residues and digestion dynamics in banana.
出处
《热带作物学报》
CSCD
北大核心
2017年第7期1331-1336,共6页
Chinese Journal of Tropical Crops
基金
现代农业产业技术体系建设专项资金(No.CARS-32-04)
中央级科研院所基本业务费项目(No.2016hzs1J007)
关键词
乙基多杀菌素
香蕉
液相色谱-串联质谱
农药残留
消解动态
Spinetoram
banana
liquid chromatography with tandem mass spectrometry
pesticide residue
dissipation dynamics
