摘要
为探讨SCoT、EST-SSR标记技术在甘草属植物遗传多样性研究的适用性,采用SCoT、EST-SSR分子标记对甘草属8自然居群的4种1变种共计14个个体进行基因组DNA的多态性检测。实验筛选出的18对SCoT通用引物共扩增出313条带,其中312条为多态性条带,平均多态率(PPB)为99.68%,平均有效等位基因数(Ne)和位点品均期望杂合度(He)等遗传参数与EST-SSR标记无显著差异。多态性检测效率高的标记为SCoT,E=17.333,Ai=21.145,高于EST-SSR,E=6.909,Ai=8.719。SCoT与EST-SSR标记聚类分析结果趋势相似,均将14份材料划分为3组,与基于形态学分类结果一致。2种标记相比,EST-SSR(0.820)产生的平均相似系数范围大于SCoT(0.785),EST-SSR标记个体间遗传差异检测能力略高于SCoT。结果表明SCoT和EST-SSR两种标记均可揭示甘草属种间与种内的遗传多样性水平以及亲缘关系,是进行甘草属植物自然群体的遗传结构、种间基因渐渗等研究的有效分子标记。其中,SCoT标记多态性高,信息量大,更适于甘草属植物种质资源的遗传多样性分析;个体间遗传差异检测上EST-SSR标记效果更佳,更利于疑难种鉴定及亲本分析。
To applied researches in germplasm resources and genetic diversity of medicinal plant in Glycy^hiza were carried out based on SCoT and EST-SSR molecular markers. Genomic DNA polymorphic comparative analysis by SCoT and EST-SSR markers in, a total of 14 individuals, are collected from 8 natural populations, including four species and one variant of Glycyrrhiza. The results showed that total of 313 bands were detected using 18 pairs of primers, among which 312 were polymorphic bands. The average percentage of polymorphic bands was 99.68%, and there were no significant difference in the average effective number of alleles（Ne） and the expected heterozygosity （He） between EST-SSR and SCoT markers. SCoT marker （E=17.333, Ai=21.145） was higher than EST-SSR （E=6.909,Ai=8.719） in assay efficiency. The systematic diagram of genetic relationship indicated that SCoT and EST-SSR markers grouped the 14 individuals into three major groups of the same. This cluster was in accord with the classical taxonomy, indicating that both methods were efficient in revealing interspecific or intraspecific genetic difference and relationship. These results indicates that SCoT markers bring a rich in polymorphism and informativeness and it would be applied more efficient than EST-SSR to germplasm analysis of the genetic diversty for Glycyrrhiza L.. Meanwhile, EST-SSR is good at detecting differences among individuals, and better for identification and parentage analysis.
作者
宋凤
陆嘉惠
韩春
牛清东
陈超南
李学禹
Song Feng Lu Jiahui Han Chun Niu Qingdong Chen Chaonan Li Xueyu(College of Life Science,Shihezi University/The Key Oasis Eco-Agriculture Laboratory of Xinjiang Production and Construction Group, Shihezi, Xinjiang 832003, China Institute of Licorice in Shihezi University, Shihezi, Xinjiang 832003, China)
出处
《石河子大学学报（自然科学版）》
CAS
2017年第2期213-219,共7页
Journal of Shihezi University(Natural Science)
基金
国家自然科学基金项目(31260042)
