摘要
[目的]优化子宫内膜腺上皮细胞的分离培养及纯化技术,以获得经济、数量多、活性好、纯度高的子宫内膜腺上皮细胞,为子宫内膜异位症的研究提供可靠的体外模型。[方法]采用复合酶重复消化、80目筛网单次过滤、低速离心、差时贴壁法处理。[结果]经诊断性刮宫获取的19例标本中,18例腺上皮细胞培养成活,1例失败;腺上皮细胞纯度高达94.2%。[结论]复合酶多次消化、80目筛网单次过滤、低速离心、差时贴壁法培养子宫内膜腺上皮细胞,可以获得经济、数量多、活性好、纯度高的细胞。
[Objective] Improvement the isolation, culture and purification technology of human endometrial glandular epithelial cells to obtain lots of the economic cells with good activity and high purity , and provide reliable model in vitro to study of endometriosis. [Methods] Repeated digestion of composite enzyme, single filtration of screen mesh with 220 ^m in diameter, low speed centrifugal and differences in time of adherent cells. [Results] The human endometrial glandular epithelial cells were successfully cultured in 18 of 19 endometrial tissues, which were obtained from the method of diagnostic curettage. The putity of human endometrial glandular epithelial cells was up to 94.2%. [Conclusion] By the methods of repeated digestion of composite enzyme, single filtration of screen mesh with 220 ^m in diameter, low speed centrifugal and differences in time of adherent cells, we can obtain lots of the economic cells with good activity and high purity.
作者
王月玲
李沛霖
李洁
王建玲
华丹丹
王倩男
王璀
WANG Yue- ling LI Pei- lin LI Jie WANG Jian- ling HUA Dan- dan WANG Qian- nan WANG Cui(l.Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China)
出处
《天津中医药大学学报》
CAS
2017年第4期291-294,共4页
Journal of Tianjin University of Traditional Chinese Medicine
基金
国家自然科学基金项目(81072837)
关键词
子宫内膜异位症
腺上皮细胞
细胞培养
endometriosis
endometrial glandular epithelial cells
cells culture
