摘要
目的研究甲基叔丁基醚(MTBE)暴露对淋巴细胞遗传毒性损伤,为建立MTBE职业人群暴露限值体系提供参考。方法人B淋巴母细胞分别用不同浓度MTBE溶液染毒24 h,彗星实验检测细胞尾部DNA百分比及Olive尾矩,Annexin V-FITC/PI双染流式细胞术检测细胞凋亡百分比。选择某炼化厂60名MTBE职业暴露工人为暴露组,55名未接触MTBE工人为对照组,微核实验检测外周血淋巴细胞微核率,彗星实验检测外周血淋巴细胞尾部DNA百分比及Olive尾矩,酶联免疫吸附试验检测外周血血浆丙二醛(MDA)、8-羟基脱氧鸟苷(8-OHd G)及谷胱甘肽-过氧化物酶(GSH-Px)水平。结果人B淋巴母细胞经10、12.5μmol/L的MTBE染毒24 h后,尾部DNA百分比、Olive尾矩和细胞凋亡百分比均高于对照组(P<0.05)。暴露组工人外周血微核阳性率为8.93%,高于对照组的3.92%,但差异无统计学意义(P>0.05);暴露组尾部DNA百分比为(15.70±7.67)%,高于对照组的(14.65±6.20)%,差异无统计学意义(P>0.05);暴露组Olive尾矩为7.00±4.94,高于对照组的3.84±1.97(P<0.01);暴露组与对照组工人血浆MDA、8-OHd G及GSH-Px水平差异均无统计学意义(P>0.05)。结论MTBE暴露可诱导人B淋巴母细胞和人外周血淋巴细胞发生遗传损伤。
Objective To investigate the genetic toxicity of methyl tert-butyl ether （MTBE） to lymphocytes and to provide reference for establishing the occupational population exposure limit of MTBE. Methods The human B lymphocytes in the logarithmic growth phase were respectively exposed to MTBE at concentrations of 0, 1.25, 2.5, 5, 10 and 12.5 Ix mol/L. The percentage of tail DNA and Olive tail moment of human B Iymphocytes were evaluated after 24 h exposure by using comet assay. Apoptosis was tested by Annexin V-FITC/PI double-staining flow cytometry. The contents of MDA and 8-OHdG, as well as GSH-Px activity were measured by ELISA kit. Sixty workers from a petrochemical factory in Zhejiang were selected as the occupational exposure population, and 55 non-occupational exposure workers were selected as the control population. And 5 mL heparin anticoagulant peripheral blood was collected, and the number of micronucleus of peripheral blood lymphocytes was measured by micronucleus test, and the percentages of tail DNA as well as Olive tail moment of peripheral blood lymphocytes were measured by comet assay. The contents of MDA and 8-OhdG and GSH-Px activity in peripheral blood plasma were measured by ELISA kit. Results After 24 h exposure, the percentage of tail DNA and Olive tail moment in human B lymphoeytes at concentrations of 10-12.5μ mol/L were significantly higher than those in the control group （P 〈 0.05） , and the percentage of early apoptosis cells was significantly increased at concentrations of 10-12.5 μmol/L （P 〈0.01） . The results of population-based study indicated no statistically significant difference in micronucleus positive rate, and the contents of MDA and 8-OhdG, and GSH-Px activity between the exposure group and the control group, but the Olive tail moment was significantly higher in the exposure group compared with the control group （P=-0.000） . Conclusion The results of vitro study showed that exposure to 10-12.5 μmol/L MTBE could cause genetic toxicity to human B lymphocytes. Olive tail moment of peripheral bh）od lymphocytes of occupational exposure workers was significantly higher than that of non-occupational exposure group.
作者
薛惠中
王禹
高明
张国珍
朱富强
汤晓萍
阮征
钱亚玲
吴南翔
XUE Hui-zhong WANG Yu GAO Ming ZHANG Guo-zhen ZHU Fu-qiang TANG Xiao-ping RUAN Zheng QIAN Ya-ling WU Nan-xiang(Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, 300013, China)
出处
《预防医学》
2017年第8期757-761,共5页
CHINA PREVENTIVE MEDICINE JOURNAL
基金
国家科技支撑项目(2014BAI12B02)
浙江省医学支撑学科-劳动卫生学(11ZC02)
关键词
甲基叔丁基醚
人B淋巴母细胞
职业暴露
遗传损伤
Methyl tere-butyl ether
Human B lymphocytes
Occupational exposure
Genetic damage
