摘要
目的观察microRNA-4463(miR-4463)在下肢动脉硬化闭塞症(ASO)患者血浆和组织中的表达变化,推测其可能意义。方法采用miRNA芯片方法筛选ASO患者和对照人群血浆中差异表达miRNA,Real time PCR技术验证ASO患者和对照人群中miR-4463的表达水平,并进行临床分期比较。靶基因预测软件分析miR-4463靶基因,Gene Ontology和KEGG数据库分析靶基因的功能和信号通路。结果与对照组相比,ASO患者血浆中有51个miRNA变化超过1.5倍(P<0.05)。miR-4463在ASO患者血浆和病变血管内膜组织中表达均显著降低,并随Fontaine分期呈逐渐下降趋势。生物信息学分析发现miR-4463靶基因与细胞迁移、脂质代谢、内吞作用等相关。结论 miR-4463参与了ASO的病程,其表达下降可能提示ASO的发生。
Aim To observe the expression of miR-4463 in the plasma and tissues of patients with arteriosclerosis obliterans of lower limbs（ ASO）. Methods miRNA microarray method was used to screen the differentially expressed miRNA in the plasma of three ASO patients and three controls. Real time PCR technique was carried out to verify the expression level of miR-4463 in the plasma and tissues of 50 patients with ASO and 50 healthy controls,and the relationship of clinical stage with miR-4463 level was analyzed. Target gene prediction software was used to predict target genes of miR-4463,and then the function and signaling pathways of predicted target genes were analyzed by Gene Ontology and KEGG pathway. Results Compared with the controls,51 miRNAs changed in the plasma of patients with ASO more than 1.5times（ P〈0.05）. The expression of miR-4463 in plasma and vascular intimal tissues of ASO patients was significantly decreased,and decreased gradually with Fontaine stage. Bioinformatics analysis revealed that the target genes of miR-4463 were related to cell polarity,migration,lipid metabolism and endocytosis. Conclusion miR-4463 is involved in the pathogenesis of ASO,and decreased miR-4463 level may indicate the occurrence of ASO.
出处
《中国动脉硬化杂志》
CAS
北大核心
2017年第8期812-817,共6页
Chinese Journal of Arteriosclerosis
基金
四川省教育厅科研项目(16ZA0179)
泸州市-泸州医学院联合课题(LZ-LY-20)
