摘要
目的观察肝细胞肝癌(hepatocellular carcinoma,HCC)、非肝脏来源肿瘤组织中精氨酸酶-1(Arginase-1,Arg-1)、磷脂酰肌醇蛋白聚糖-3(Glypican-3,GPC-3)的表达及在病理诊断和鉴别诊断中的作用。方法收集156例HCC、5例胆管细胞癌、20例肝脏转移癌和18例其他部位恶性肿瘤,应用免疫组化En Vision法检测两种蛋白的表达。结果 Arg-1在HCC及非肝脏来源肿瘤中的阳性率分别为93.6%(146/156)和0(0/43),在HCC中的阳性率随着肿瘤分化程度的降低而减少(r=-0.264,P=0.001);GPC-3在HCC及非肝脏来源肿瘤中的阳性率分别为90.4%(141/156)和14.0%(6/43),在HCC中的阳性率随着肿瘤分化程度的降低而增加(r=0.179,P=0.026)。Arg-1和GPC-3的敏感性、特异性、阳性预测值和阴性预测值分别为93.6%、100%、100%、81.1%和90.4%、86.0%、96.0%、71.2%。结论 Arg-1是敏感和特异的肝细胞标志物,对鉴别肝脏源性和非肝脏源性肿瘤有显著优势;GPC-3是诊断HCC特异性较高的标志物,尤其在低分化HCC的鉴别诊断中更有优势;联合应用Arg-1和GPC-3,对于HCC与肝脏转移癌的诊断与鉴别诊断具有重要意义。
Purpose To evaluate the expression of Argi- nine-1 （Arg-1） and Glypican-3 （GPC-3） in hepatocellular car- cinoma （HCC） and non-hepatoeellular carcinoma, as well as to summarize the related researches. Methods 156 cases of HCC, 5 cases of eholangiocareinoma, 20 cases of metastatic ad- enocarcinoma and 18 cases of other types of tumors were stud- ied. Immunohistochemical study for Arg-1 and GPC-3 was per- formed on the formalin-fixed and paraffin-embedded tumor tis- sues. Results The positive expression rates of Arg-1 in HCC and non-hepatocellular carcinoma were 93.6% （146/156） and 0 （0/43）, respectively, meanwhile the expression rate de- creased with decreasing of differentiation （ r = - 0. 264, P = 0. 001 ）. GPC-3 expression was observed in 141 of 156 cases ofHCC （90.4%） and 6 of 43 cases of non-hepatocellular carcino- ma （14%）, and the expression rate increased with decreasing of differentiation （r =0. 179, P =0. 026）. The sensitivity, spe- cificity, positive predictive value and negative predictive value of Arg-1 and GPC-3 in distinguishing HCC from non-hepatocellular carcinoma were 93.6% , 100% , 100% , 81.1% and 90. 4% , 86%, 96. 0%, 71.2%, respectively. Conclusion Arg-1 is a sensitive and specific marker of hepatocytes. The application of Arg-1 and GPC-3 is of great significance in diagnosis of HCC and liver metastatic adenocarcinoma.
出处
《临床与实验病理学杂志》
CSCD
北大核心
2017年第8期841-846,共6页
Chinese Journal of Clinical and Experimental Pathology
