摘要
采用高效液相色谱(HPLC)法测定牡丹籽油中游离型、结合型α-亚麻酸、亚油酸及油酸的含量。检测条件为Agilent C18高效色谱柱(3.0 mm×50 mm,2.7μm),流动相乙腈-0.1%磷酸水溶液(体积比85∶15),流速1 mL/min,检测波长203 nm,柱温35℃,进样量20μL。结果表明,该方法精密度、重复性、稳定性良好。α-亚麻酸在41.4~207μg/mL范围内,其峰面积与质量浓度呈良好的线性关系;亚油酸在20.2~101μg/mL范围内,其峰面积与质量浓度呈良好的线性关系;油酸在14.9~74.6μg/mL范围内,其峰面积与质量浓度呈良好的线性关系。牡丹籽油样品中,游离型α-亚麻酸、亚油酸和油酸的平均含量分别为4.47、1.89 mg/mL和0.92 mg/mL,结合型α-亚麻酸、亚油酸和油酸的平均含量分别为437、237 mg/mL和156 mg/mL。建立的方法具有分离效率高、选择性好和条件温和的优点。
The contents of free and conjugated α- linolenic acid, linoleic acid and oleic acid in Paeonia suticosa Andr. seed oil were determined by HPLC. The detection conditions were as follows： Agilent C18 column （3.0 mm -0 mm, 2.7μm）, mobile phase acetonitrile -0.1% phosphoric acid solution （volume ratio 85：15 ）, flow rate 1 mL/min, detection wavelength 203 nm, column temperature 35℃ and injection volume 20 μL. The results showed that the method had good precision, repeatability and stability. Good linear relationships of α- linolenic acid, linoleic acid and oleic acid were achieved in the mass concentration ranges of 41.4 -207 μg/mL, 20.2 - 101μg/mL and 14.9 -74.6μg/mL, respec-tively. The average contents of free α - linolenic acid, linoleic acid and oleic acid were 4.47,1.89 mg/mL and 0.92 mg,/mL respectively, and the average contents of conjugated α -linolenic acid, linoleic acid and oleic acid were 437,237 mg/mL and 156 mg/mL respectively in Paeonia suffrutico- sa Andr. seed oil. The methods had the advanta- ges of high separation efficiency, good selectivity and mild conditions.
出处
《中国油脂》
CAS
CSCD
北大核心
2017年第8期140-144,共5页
China Oils and Fats
基金
2016年陕西省教育厅专项科研计划项目(16JK1650)
陕西省2014年省级大学生创新创业训练计划项目(1644)
西安医学院2015年大学生创新创业训练计划项目(2015DXS1-18)
关键词
牡丹籽油
Α-亚麻酸
亚油酸
油酸
高效液相色谱法
Paeonia suffruticosa Andr. seed oil
α - linolenic acid
linoleic acid
oleic acid
HPLC
