摘要
目的 :比较菊芋蛋白两种提取方法的蛋白质提取率,并做菊芋蛋白降血糖研究。方法 :菊芋采用丙酮沉淀法和硫酸铵盐析法提取蛋白质,以蛋白质含量为指标,优选蛋白质提取率高的提取方法,并通过小鼠体内降血糖实验,评价菊芋蛋白的降血糖效果。结果:丙酮沉淀法蛋白提取率为11.30%,硫酸铵盐析法提取率为10.80%;降糖作用研究表明,菊芋蛋白高剂量组降血糖作用明显。结论:丙酮沉淀法提取率高,菊芋蛋白具有降血糖的效果。
Objective：To compare the extraction rate of two extraction methods for Helianthus tuberosus pro- tein,and to evaluate its antidiabetic effect. Methods Acetone precipitation and ammonium sulfate salting-out methods were applied for protein extraction,and protein content was applied as the index to optimize the extraction method,and the hypoglycemic activity was evaluated in the mouse. Results：The protein extraction rate by acetone precipitation was 11.30%,while that by ammonium sulfate salting-out method was 10.80%. The high dose of Helianthus tuberosus protein showed obvious hypoglycemic activity. Conclusion：Acetone sedimentation method is of high extraction rate,and Helianthus tuberosus protein is of hypoglycemic activity.
出处
《山东中医杂志》
2017年第8期714-716,724,共4页
Shandong Journal of Traditional Chinese Medicine
关键词
菊芋蛋白
丙酮沉淀法
硫酸铵盐析法
蛋白质电泳
紫外测含量
降血糖
Helianthus tuberosus protein
acetone precipitation
ammonium sulfate salting-out method
protein electrophoresis
ultraviolet spectrophotometry
hypoglycemic activity
