摘要
目的探讨阿尔茨海默病相关神经丝蛋白(AD7c-NTP)表达水平的变化在阿尔茨海默病(AD)早期诊断和病情评估中的意义。方法选取AD组58例(轻度AD组26例、中重度AD组32例),轻度认知功能障碍患者(MCI组)52例和对照组62例,采用酶联免疫吸附法(ELLSA)测定各组患者尿AD7c-NTP值,通过MMSE量表评定并行统计学分析。结果不同受试组尿AD7c-NTP表达水平差异具有统计学意义(P<0.05),其中AD组和MCI组尿AD7c-NTP水平均高于对照组,AD组高于MCI组,中重度AD组高于轻度AD组。经Spearman秩相关分析,轻度AD组、中重度AD组、MCI组尿AD7c-NTP表达水平与MMSE评分呈负相关,与病程呈正相关。ROC曲线所显示的曲线下面积为0.952(95%CI:0.913~0.992,P=0.000),当尿AD7c-NTP最佳临界值为1.76 ng/ml时,诊断AD的灵敏度和特异度分别是93.1%和90.3%。结论尿AD7c-NTP的表达水平对于AD的早期诊断和病情评估具有重要的参考价值。
Objective Objective to investigate the value of urine AD7c-NTP expression in early diagnosis and evaluation of Alzheimer＇s disease. Methods Select 58 cases of Alzheimer＇s disease（ group AD）（ mild AD group of 26 cases,moderate to severe AD group of 32 cases）,mild cognitive impairment（ MCI group） of 52 cases,and control group of 62 cases,Enzyme-Linked Immuno Sorbent Assay（ ELLSA） was used to detect the expression of unrine AD7c-NTP,scoring by MMSE scale and collecting and analyzing the urine AD7c-NTP expression level. Results The results showed that there was a obvious statistical significance（ P〈0. 05） in the expression level of urine AD7c-NTP among the different test groups. Besides,the expression level of urine AD7c-NTP of AD group and the MCI group were all exceeding the control group. The urine AD7c-NTP level of AD group was higher than that of MCI group,the urine AD7c-NTP level of moderate and severe AD group was higher than that of mild AD group. Analyzed by the spearman rank correlation,the group of mild AD group,the moderate-severe AD group and MCI group had a negative correlation with the level of MMSE,and a positive correlation with the course of the disease. Area under the ROC curve was 0. 952（ 95% CI： 0. 913 - 0. 992,P = 0. 000）,when the optimum critical value get 1. 76ng/ml,sensitivity and specificity for diagnosing AD was 93. 1% and 90. 3%. Conclusion The expression level of AD7c-NTP in urine has an important reference value for the early diagnosis and assessment of Alzheimer＇s disease.
出处
《中风与神经疾病杂志》
北大核心
2017年第8期684-686,共3页
Journal of Apoplexy and Nervous Diseases
基金
潍坊市软科学研究计划项目(No.2016RKX035)
