摘要
利用^(60)Co-γ射线对红掌‘阿拉巴马’的愈伤组织进行辐射,根据再生植株的叶色和花色变化筛选出突变体,并对突变体与亲本进行形态学、细胞学及分子水平的差异分析。结果表明:红掌‘阿拉巴马’愈伤组织的半致死剂量接近30Gy。当辐射剂量为30Gy时,再生植株M_1代、M_2代的性状一致,染色体数目与亲本一致2n=2x=30,仅有RAPD-751引物(序列:CCCACCACAC)条带与亲本有差异,并且能够稳定遗传,从而成功诱变出红掌突变体,这对提高红掌的育种效率具有重要的理论和实践意义。
Calluses fromAnthurium andraeanum(Alabama)were exposed to ^(60)Co-γrays to induce mutation on the tissue.Based on the color changes on the leaves and flowers,resulting mutants were selected among the regenerated plants.Subsequently,the morphological,cellular and molecular properties of the selected mutants and their parents were analyzed.The half-lethal dose of ^(60)Coγ-ray on the callus was found to be close to 30 Gy.Treated by the dosage,the mutated M1 and M2generations showed similar traits.They had a chromosome number same as that of the parents.But,they differed from the parents in RAPD on the D-751 primer that had a sequence of CCCACCACAC.Since the mutants could consistently pass on the inherited genes to the regenerated protoplasts,theoretically,successful breeding of mutated plants fromA.andraeanumby irradiation treatment could be expected in practice.
出处
《福建农业学报》
CAS
北大核心
2017年第7期710-714,共5页
Fujian Journal of Agricultural Sciences
基金
福建省林业厅项目(闽林2015S0040)
