摘要
研究了以豆粕(添加2%葡萄糖)为基质的植物乳杆菌固态发酵菌剂制备的最佳条件。以干燥后活菌数为指标,通过单因素和Box-Behnken响应面实验设计对发酵工艺进行优化,对豆粕发酵前后的主要营养成分及抗营养因子含量进行测定,并最终确定了烘干条件。结果表明,在发酵温度30.6℃、接种量4.45%、加水比1∶0.61、发酵时间46.40 h时,活菌数为最高达到9.94 lg(CFU/g);粗蛋白、酸溶蛋白、游离氨基酸总量和蛋白质消化率较发酵前分别提高了4.55%、1.24%、50.47 mg/g和4.21%;胰蛋白酶抑制因子、脲酶和植酸较发酵前分别降低了94.59%、91.64%和30.22%;最佳干燥条件为热风干燥50℃、4 h,菌剂活菌总数为9.88 lg(CFU/g)。
Research herein was focused on the best condition for preparation of Lactobacillus plantarum agent with soybean meal containing 2% glucose. The number of viable cells after drying was used as an indicator to investigate the production process. The production process was optimized through one-factor experiment combined with response surface methodology. After fermentation, content of crude protein, acid soluble protein, free amino acids, trypsin inhibitor, urease, and phytic acid, as well as protein digestibility were determined. Results showed that Lactobacillus plantarum achieved the highest biomass after incubation at 30.6℃ for 46.40 h, with substrate to water ratio of 1：0.61 and inoculum concentration of 4.45%. The content of crude protein, acid soluble protein, and total free amino acids, and protein digestibility were increased 4.55% , 1.24% , 50.47 mg/g and 4.21% respectively. The content of trypsin inhibitor, urease, and phytic acid were decreased 94.59% , 91.64% , and 30.22% , respectively. The number of Lactobacillus plantarum could reach 9.88 lg（CFU/g） under the best drying condition of 50℃ hot air for 4 h.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2017年第8期127-133,共7页
Food and Fermentation Industries
基金
江西省主要学科学术和技术带头人培养计划(20133BCB22001)
江西省重大科技专项计划(赣财教字[2014]104号)
"赣鄱英才555工程"领军人才培养计划(赣才字[2013]2号)
江西省蔬菜产业技术体系岗位专家项目(JXARS-06)
关键词
植物乳杆菌
豆粕
固态发酵
Box-Behnken试验
Lactobacillus plantarum
soybean meal
solid-state fermentation
Box-Behnken experimental design
