摘要
【目的】比较不同黄芪剂量的补中益气汤对脾虚气陷型内痔Ⅲ期患者的治疗效果及对内痔组织fibulin-3蛋白表达的影响,分析不同剂量的黄芪在补中益气汤中的作用及可能机制。【方法】将55例患者随机分为对照组(仅给予手术,不给予补中益气汤干预治疗,N=15)、中药1组(手术治疗前给予黄芪剂量为20 g的补中益气汤干预治疗,N=20)、中药2组(手术治疗前给予黄芪剂量为50 g的补中益气汤干预治疗,N=20)。评价补中益气汤治疗前后Ⅲ期内痔患者肛门坠胀、脱垂症状评分,并采用免疫蛋白印迹法(Western blot)检测内痔组织中fibulin-3蛋白的表达。【结果】经补中益气汤干预后,中药1、2组患者肛门坠胀、脱垂症状均有明显改善(P<0.05),且中药2组对肛门坠胀症状的改善作用优于中药1组(P<0.05);与对照组比较,中药1、2组患者内痔组织的fibulin-3蛋白表达均明显升高(P<0.05),中药2组内庤组织fibulin-3蛋白表达较中药1组亦有所升高,但差异无统计学意义(P>0.05)。【结论】大剂量黄芪的补中益气汤对脱垂性痔的治疗效果优于小剂量黄芪的补中益气汤,但产生其差异的机制与对fibulin-3蛋白表达的促进作用程度关系不明显。
Objective To compare the effect of Buzhong Yiqi Decoction（BYD） containing different doses of Radix Astragali on fibulin-3 expression in the hemorrhoid tissues of stage Ⅲ internal hemorrhoids patients with spleen deficiency and sinking of qi,and to evaluate its therapeutic efficacy and possible mechanism. Methods Fifty-five qualified patients were randomly divided into control group（N = 15）,Chinese medicine group 1（N =20）,and Chinese medicine group 2（N = 20）. All of the 3 groups were treated with operation,and additionally,Chinese medicine group 1 was given BYD containing Radix Astragali 20 g,and Chinese medicine group 2 was given BYD containing Radix Astragali 50 g orally after operation. The scores of anal pendant expansion and anal prolapse were evaluated, and the expression level of fibulin-3 in the hemorrhoid tissues was detected by Western blot method. Results After treatment,the symptoms of anal pendant expansion and anal prolapse were improved in the 2 Chinese medicine groups（P〈 0.05）, and the improvement of anal pendant expansion symptom in Chinese medicine group 2 was superior to that of Chinese medicine group 1（P〈 0.05）. Compared withthe control group,the expression level of fibulin-3 in the hemorrhoid tissues of the two Chinese medicine groups was increased（P 〈0.05）; the expression level of Chinese medicine group 2 showed an increasing trend as compared with that of Chinese medicine group 1,but the difference was statistically insignificant（P〉 0.05）.Conclusion BYD with large dose of Radix Astragali exerts stronger therapeutic efficacy for the treatment of prolapsed hemorrhoids than BYD with small dose of Radix Astragali, and its therapeutic mechanism has no obvious relation with promoting the increase of fibulin-3 expression.
作者
金鑫
万星阳
黄丹丹
李玉英
孙锋
JIN Xin WAN Xing-Yang HUANG Dan-Dan LI Yu-Ying SUN Feng(Dept. of Anorectum, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405 Guangdong, China Surgery Dept. of Anorectum, the Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655 Guangdong, China)
出处
《广州中医药大学学报》
CAS
2017年第5期640-644,共5页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(编号:81302976)
广东省中医药管理局基金资助项目(编号:20152123)
关键词
黄芪
补中益气汤
内痔
抗衰老蛋白
Radix Astragali
Buzhong Yiqi Decoction
internal hemorrhoids
anti-aging proteins
