摘要
目的通过检测青海地区胃癌组织、癌旁组织及正常胃组织中P53、P21、PTEN蛋白表达水平并分析其相互关系,为青海地区胃癌的诊断和预后的评估提供依据。方法使用免疫组织化学法检测78例胃癌组织、癌旁组织及30例正常胃组织中P53、P21、PTEN的蛋白表达水平并行相关分析。结果胃癌组织中P53蛋白表达阳性率为44.87%,P21蛋白表达阳性率为52.56%,PTEN蛋白表达阳性率为47.44%。与正常胃组织相比,差异均有统计学意义(P<0.05)。胃癌组织中,P53表达与患者年龄、性别、淋巴结转移、浸润深度及PTNM分期无关(P>0.05),与肿瘤分化程度有关(P<0.05)。P21表达与患者年龄、性别、浸润深度无关,与肿瘤分化程度、淋巴结转移及PTNM分期有关(P<0.05)。PTEN表达与患者年龄、性别、分化程度、浸润深度无关,与肿瘤淋巴结转移和PTNM分期有关(P<0.05)。胃癌组织中P53与P21表达存在关联,其列联系数为0.323(P<0.05)。P53与PTEN,P21与PTEN之间均存在一定的相关性,但无统计学意义(P>0.05)。结论青海地区患者胃癌组织中P53与P21表达存在关联。P53与PTEN、P21与PTEN相关,但无统计学意义。
Objective To support clinical diagnosis of gastric carcinoma by examining the protein levels of P53,P21 and PTEN in gastric carcinoma tissues,the tissues adjacent to carcinoma and normal gastric tissues in Qinghai Area.Methods 78 cases of protein levels of P53, P21 and PTEN in gastric carcinoma tissues, the tissue ad- jacent to carcinoma and normal gastric tissues were detected by immunohistochemical method, and analyzed their correlations.Results The protein expressions of P53 ,P21 and PTEN in gastric carcinoma were all higher than those of normal gastric tissues,the positive rates were 44.87% ,52.56% and 47.44% ,respectively,and the difference was statistically significant（P〈0.05）.The positive expressions rate of P53 protein in gastric cancer was correlated with differentiation,the difference was statistically significant（P〈0.05） .The positive expressions rate of P53 protein in gastric cancer has no correlation with age, gender,lymph node metastasis, invasion depth and the PTNM staging, the difference was not statistically significant（P〉0.05）.The positive expressions rate of P21 protein in gastric cancer was correlated with lymph node metastasis,invasion depth and the PTNM staging, the difference was statistically sig- nificant（P〈0.05） .The positive expressions rate of P21 protein in gastric cancer has no correlation with age, gender, differentiation, the difference was not statistically significant （P〉0.05）.The positive expressions rate of PTEN pro- tein in gastric cancer was correlated with lymph node metastasis and the PTNM staging, the difference was statisti- cally significant（P〈0.05）.The positive expressions rate of PTEN protein in gastric cancer has no correlation with age, gender, differentiation, invasion depth, the difference was not statistically significant （ P〉 0.05 ）. The expression of P53 was correlated with P21, the coefficient of contingency was 0.323 （ P〈0.05 ）, the expression of P53 and PTEN ,P21 and PTEN has no statistical correlation （P〉0.05）. Conclusion The expression of P53 was correlated with P21 ,the expression of P53 and PTEN,P21 and PTEN has no statistical correlation in Qinghai Area.
作者
薛晓红
王亚平
朱智勇
李晓林
马颖才
XUE Xiao-hong WANG Ya-ping ZHU Zhi-yong LI Xiao-lin MA Ying-cai(Digestive Department of Qinghai Provincial People＇s Hospital, Xining, Qinghai 810007)
出处
《中国高原医学与生物学杂志》
CAS
2017年第3期188-193,共6页
Journal of Chinese High Altitude Medicine & Biology
基金
青海省科学技术厅科技促进新农村计划(2013-N-526)
