摘要
目的 确定禽流感病毒H7N9神经氨酸酶的优势线性B细胞抗原位点.方法 利用生物信息学软件DNAStar中的Protean程序分析禽流感病毒H7N9神经氨酸酶氨基酸序列的亲水性、抗原性和表面可及性,计算不同区段上述指数的加权平均值,预测出潜在的优势线性B细胞抗原位点并进行人工合成.利用多肽-酶联免疫吸附试验（多肽-ELISA）检测各预测片段与明确感染H7N9患者血清的反应性,同时以未感染H7N9的正常人血清作为阴性对照.结果 根据抗原性、亲水性和表面可及性指数均较高的特点,共预测出7个潜在的神经氨酸酶线性B细胞抗原位点,分别为A、B、C、D、E、F和G多肽-ELISA实验结果证实各预测表位均与H7N9患者血清发生阳性反应.结论 成功预测及确定了禽流感病毒H7N9神经氨酸酶优势线性B细胞抗原位点,为抗原分子特异性研究和抗体制备奠定了基础.
Objective To identify immunodominant linear B cell epitopes in neuraminidase of avian influenza virus H7N9.Methods By using protean algorithms of bioinformatic software DNAStar,antigenicity,hydrophilicity and surface probability of the H7N9 neuraminidase sequence was analyzed and their corresponding average indexes were calculated.Multiple regions containing potential linear B cell epitopes were predicted.Corresponding peptides were synthesized artificially and used in peptide-ELISA individually to check their reactivity to confirmed H7N9 positive human sera,and H7N9 negative human sera was used as control.Results Seven potential linear B cell epitopes,namely A to G,were predicted according to their relatively strong antigenicity,hydrophilicity and surface probability.All corresponding synthetic peptides reacted strongly with H7N9 positive sera.Conclusions Immunodominant linear B cell epitopes in neuraminidase of H7N9 were successfully predicted and confirmed.It will facilitate to clarify molecular basis of the antigen specificity and to make respective antibodies.
作者
高孟
谢珲
姜立民
高丽美
倪红霞
罗永能
Gao Meng Xie Hui Jiang Limin Gao Limei Ni Hongxia Luo Yongneng(Institute of Viral Diseases, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China（ Gao M, Xie H, Jiang LM, Gao LM, Luo YN Ningbo Center for Disease Control and Prevention, Ningbo 310010, China （ Ni HX)
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2017年第4期287-290,共4页
Chinese Journal of Experimental and Clinical Virology
基金
浙江省医药卫生科技计划（2014KYA039）
浙江省重点实验室科技计划（2008F3022）
