摘要
将拟南芥AtCBF1、AtCBF2和AtCBF3基因簇完整地导入番茄基因组,以期修正番茄CBF冷应答系统的遗传缺陷,提高番茄的耐寒性.结果显示,转基因番茄植株中外源AtCBF1、AtCBF2和AtCBF3基因能被4℃低温诱导表达,6h后相对表达量均提高大约200倍;低温胁迫下,转基因番茄的细胞膜透性和丙二醛(MDA)含量的增加量显著低于野生型对照,而超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性增强幅度显著高于野生型材料,从而使转基因番茄的耐寒性得到显著提高.
Tomato,as a kind of cold sensitive plant,whose CBFcold response system is part of the conservative,lacks the perfect cold adaptation mechanism as Arabidopsis.In order to revise the genetic defect of the CBF cold response system of tomato and improve its cold tolerance,the AtCBF1,AtCBF2 and AtCBF3 gene clusters of Arabidopsis were completely transferred into the tomato genome.The result showed that the exogenous AtCBF1,AtCBF2 and AtCBF3 genes could be induced by low temperature at4℃ in the transgenic tomato plants and their relative expression increased about 200 times in 6h.Under low temperature stress,the increase in cell membrane permeability and MDA content of the transgenic tomato plants was significantly lower than those of the wild type,while the increment of SOD,POD and CAT activities in them was significantly higher than that of the wild type.Therefore the cold tolerance of the transgenic tomato was significantly improved.
作者
葛歌
金新开
沈辉
李小寒
潘宇
李金华
苏承刚
张兴国
GE Ge JIN Xin-kai SHEN Hui LI Xiao-han PAN Yu LI Jin-hua SU Cheng-gang ZHANG Xing-guo(School of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China , Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400716, China)
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2017年第9期34-41,共8页
Journal of Southwest University(Natural Science Edition)
基金
科技部"863"基金项目(2010AA10060705)
