摘要
目的探究肝源性溃疡中胃黏膜组织血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)的表达,分析其与病情进展及患者并发症发生率的关系。方法临床选取2014年7月至2016年5月133例肝硬化伴门静脉高压合并胃或十二指肠溃疡患者,内镜下活检溃疡边缘及正常黏膜组织为对照。采用反转录-聚合酶链反应(RT-PCR)技术检测正常胃黏膜(133例)、胃溃疡活动期(GA期52例)、胃溃疡愈合期(GH期45例)、胃溃疡瘢痕期(GS期36例)患者黏膜组织VEGF mRNA、bFGF mRNA的表达情况,并与患者的并发症发生率行Spearman秩相关分析。结果溃疡边缘组织VEGF mRNA、bFGF mRNA的表达量显著高于正常黏膜组织,差异有统计学意义(P<0.05);GH、GS期患者VEGF mRNA的表达量明显高于GA期患者,差异有统计学意义(P<0.05),GS期患者bFGF mRNA的表达量明显高于GA期患者,差异有统计学意义(P<0.05);Spearman秩相关分析结果示VEGF mRNA、bFGF mRNA的表达量与患者溃疡并发症发生率呈负相关(rs=-0.672,-0.508,P<0.05)。结论VEGF mRNA、bFGF mRNA在肝源性溃疡的修复过程中可能发挥重要的促进作用,是潜在的临床标志物。
Objective To investigate the expression of vascular endothelial growth factor（VEGF）and basic fibroblast growth factor（bFGF）in gastric mucosa of patients with hepatogenic ulcer,and to analysis the relationship between them and the incidence of complications and disease progression in patients.Methods During July 2014 to May 2016,133 cases of patients with hepatocirrhosis and portal hypertension associated with gastric or duodenal ulcer were selected as study object.Marginal tissue of ulcer were collected,and normal mucosa tissue were collected as control group.Reverse transcription-polymerase chain reaction（RT-PCR）detect the expression of VEGF mRNA and bFGF mRNA in 133 cases of normal gastric mucosa and 133 cases of marginal tissue of ulcer,including 52 cases of gastric ulcer activity period（GA）,45 cases of gastric ulcer healing period（GH）and 36 cases of gastric ulcer scar period（GS）.Spearman correlation coefficient analysis the correlation between the expression of VEGF mRNA and bFGF mRNA and complication rate.Results The expression of VEGF mRNA and bFGF mRNA in ulcer marginal tissues was significantly higher than that in normal mucosa tissues,the difference was statistically significant（P0.05）.The expression of VEGF mRNA in GH and GS of patients was significantly higher than that in GA patients,the difference was statistically significant（P0.05）,the expression of bFGF mRNA in GS of patients was significantly higher than that of GA patients,the difference was statistically significant（P0.05）.Spearman correlation analysis showed VEGF mRNA and bFGF mRNA was negatively correlated with incidence rate of ulcer complications（rs=-0.672,-0.508,P0.05）.Conclusion bFGF mRNA and VEGF mRNA may play an important role in the repair of hepatogenic ulcer.It is a potential clinical marker.
出处
《国际检验医学杂志》
CAS
2017年第18期2579-2581,共3页
International Journal of Laboratory Medicine
