摘要
本研究旨在应用胶体金免疫层析技术,结合胶体金定量读数仪研制出一种快速定量检测谷物和饲料中黄曲霉毒素B_1含量的胶体金快速定量检测试剂盒。该试剂盒以胶体金标记高特异性单抗,与偶联抗原进行正交试验,确定适宜条件,同时通过与对照线和试验线的颜色对比,应用胶体金定量读数仪,对样本中黄曲霉毒素B_1含量进行定量检测。结果表明,黄曲霉毒素B1胶体金快速定量检测试剂盒检测方法简便、快速、稳定性好,且可得出具体数据,避免了人肉眼观察的差异;与常见霉菌毒素无交叉反应,样品检测结果与高效液相色谱法检测结果的相对误差在20%以内。由此可见,本试验研制的黄曲霉毒素B_1胶体金快速定量检测试剂盒可用于谷物和饲料中黄曲霉毒素B_1含量的快速定量检测。
This experiment w as conducted to develop a colloidal gold rapid and quantitative test kit for detected aflatoxin B_1 content in grain and feed by using colloidal gold immunochromatography technology and colloidal gold quantitative test instrument. The kit using colloidal gold-labeled highly specific monoclonal antibody and conjugated-antigen in an orthogonal experiment to find the optimum condition,meanw hile,by compared the color of the control line and the test line,using the colloidal gold quantitative test instrument to detected the aflatoxin B_1 content in samples. The results show ed that the aflatoxin B_1 colloidal gold rapid and quantitative test kit w as simple,rapid and stability,the specific data could be obtained,avoided the differences observed by naked eyes. This kit had no cross reaction w ith common mycotoxin,the relative error of aflatoxin B_1 colloidal gold rapid and quantitative test kit detection results and high performance liquid chromatography detection results w as less than 20%. In conclusion,this aflatoxin B_1 colloidal gold rapid and quantitative test kit w hich developed by the experiment can be used for testing the aflatoxin B_1 content in grain and feed rapidly and quantitative.[Chinese Journal of Animal Nutrition,2017,29( 10) : 3703-3709]
出处
《动物营养学报》
CAS
CSCD
北大核心
2017年第10期3703-3709,共7页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
重庆市农发资金项目(16411)
