摘要
目的比较实时荧光核酸恒温扩增技术(SAT)和液体培养法在解脲脲原体(UU)检测中的诊断价值,以选择更为准确、快速、实用的临床检测方法。方法采集本院就诊的不孕不育对象200例的分泌物2份拭子样本,分别用于液体培养法和SAT法检测。结果液体培养法阳性率为60%,SAT法阳性率为47%,培养法阳性率高于SAT法,女性UU阳性率显著高于男性,26例结果不一致中有11例培养阴性SAT阳性,15例培养阳性而SAT阴性,SAT法的敏感性(100%)和特异性(93.5%)均高于培养法。结论解脲脲原体在不孕不育人群中感染率较高,加强就诊对象UU的筛查、诊治和预防,确保优生优育。SAT技术简便、快速、精准,具有较高的临床实用性,是适合实验室诊断的检测方法 。
Objective To evaluate the diagnostic value of real-time fluorescence nucleic acid thermostatic expansion technology（SAT） and liquid culture method in the detection of Ureaplasma Urealyticum（UU）,and select a more accurate,rapid and practical clinical testing method. Methods Swab and secretion were collected from 200 sterile patients in our hospital. Swab and secretion were tested by SAT and liquid culture method respectively. Results The positive rate of liquid culture was 60%,while the positive rate of SAT method was 47%. The positive rate of culture was higher than the SAT method. Female UU positive rate is significantly higher than male. The results of 26 patients were inconsistent between SAT and liquid culture method. 11 cases were negative tested by liquid culture method but positive by SAT,and 15 cases were positive tested by liquid culture method but negative by SAT. The sensitivity（100%） and specificity（93.5%） of SAT method were higher than culture method. Conclusion The infection rate of urea mycoplasma is higher in the population of infertile people. Thus we should strengthen screening,diagnosis and prevention of UU,and ensure eugenics. The SAT is a simple,fast and precise technique for detection of UU,and has high clinical practicality,which is suitable for clinical laboratory diagnosis.
出处
《实验与检验医学》
CAS
2017年第5期673-676,共4页
Experimental and Laboratory Medicine
基金
河南省属科研院所基本科研业务费专项资金项目(编号:JBKY2017003)
