摘要
目的探讨^(60)Co γ射线辐照对去白细胞悬浮红细胞质量的影响。方法将30袋去白细胞悬浮红细胞随机分为辐照组(n=15)和对照组(n=15),辐照组血袋经^(60)Co γ射线辐照,对照组血袋未经^(60)Co γ射线辐照,辐照后分别检测2组去白细胞悬浮红细胞中的细胞外K+、Na+、游离血红蛋白(FHb)、三磷腺苷(ATP)、2,3-二磷酸甘油酸(2,3-DPG)含量和p H值。结果辐照组去白细胞悬浮红细胞血袋中的K+、Na+、FHb、ATP、2,3-DPG含量和p H值分别为(5.20±0.37)mmol·L^(-1)、(140.22±0.05)mmol·L^(-1)、(0.44±0.04)mg·L^(-1)、(5.18±0.13)μmol·L^(-1)、(3.34±0.30)mmol·L^(-1)和6.85±0.02,对照组去白细胞悬浮红细胞血袋中的K+、Na+、FHb、ATP、2,3-DPG含量和p H值分别为(5.00±0.40)mmol·L^(-1)、(140.47±0.83)mmol·L^(-1)、(0.42±0.08)mg·L^(-1)、(5.22±0.06)μmol·L^(-1)、(3.56±0.52)mmol·L^(-1)和6.84±0.05,2组比较差异均无统计学意义(t=1.93、-1.67、-1.64、-2.02、1.77、1.82,P>0.05)。结论^(60)Co γ射线辐照对去白细胞悬浮红细胞的活性和功能无显著影响。
Objective To investigate the effect of ^60Coγ-ray irradiation on the quality of theleukocyte-filtrated red blood cells suspension.Methods Thirty bags of leukocyte-filtrated red blood cells were randomly divided into irradiation group(n =15) which was given ^60Coγ-ray irradiation and control group(n =15) which was not given ^60Coγ-ray irradiation.The content of extracellular K^+,Na ^+,free hemoglobin (FHb),adenosine triphosphate (ATP) and 2,3-diphosphoglyceric acid (2,3-DPG) and pH value of the leukocyte-filtrated red blood cells were detected in the two groups.Results The content of K^+,Na^+,FHb,ATP,2,3-DPG and pH value in irradiation group was (5.20 ± 0.37) mmol · L^-1,(140.22 ± 0.05) mmol · L^-1,(0.44 ±0.04) mg· L^-1,(5.18 ± 0.13)μmol· L^-1,(3.34±0.30) mmol· L^-1 and 6.85±0.02;the content of K +,Na +,FHb,ATP,2,3-DPG and pH value in the control group was(5.00 ± 0.40)mmol · L^-1,(140.47 ± 0.83)mmol · L^-1,(0.42 ± 0.08) mg · L^-1,(5.22 ± 0.06) μmol · L^-1,(3.56 ± 0.52) mmol · L^-1 and 6.84 ± 0.05;there was no statistic difference in the content of K ^+,Na ^+,FHb,ATP,2,3-DPG,and pH value between the two groups(t =1.93,-1.67,-1.64,-2.02,1.77,1.82;P 〉 0.05).Conclusion ^60Coγ-ray irradiation of the leukocyte-filtrated red blood cells can not effect the activity and function of red blood cells.
出处
《新乡医学院学报》
CAS
2017年第9期813-814,818,共3页
Journal of Xinxiang Medical University
关键词
Γ射线
去白细胞悬浮红细胞
辐照
淋巴细胞
输血相关性移植物抗宿主病
γ-ray
leukocyte-filtrated red blood cells suspension
irradiation
lymphocyte
transfusion associated graft versus host disease