摘要
目的探讨尿蛋白分析[尿α_1-微球蛋白(α_1-MG)、β_2-微球蛋白(β_2-MG)、微量白蛋白(MA)、尿总蛋白(UTP)、N-乙酰-β-D-氨基葡萄糖苷酶(NAG)]对于诊断早期肾损伤的临床价值。方法收集病理上无明显肾小球、肾小管病变且尿常规检查正常但可能存在早期肾损伤的高血压病、糖尿病患者的尿液标本80例,同期收集健康体检尿液标本80例为健康对照组。检测各组α_1-MG、β_2-MG、MA、UTP、NAG的含量,并对结果进行统计学分析。结果当出现早期肾损伤(如高血压、糖尿病)尿α_1-MG、β_2-MG、MA、UTP、NAG均有不同程度的升高,5项检测指标之间具有较好的相关性,且与健康对照组相比差异具有统计学意义[α_1-MG(mg/L):19.7±1.4比5.3±1.6,β_2-MG(mg/L):10.8±0.04比1.2±0.03,MA(mg/L):56.3±6.7比8.6±4.2,UTP(g/L):0.395±0.097比0.069±0.028,NAG(U/L):78.4±7.6比17.9±5.1,均P<0.01]。结论对尿α_1-MG、β_2-MG、MA、UTP、NAG进行联合检测可以为肾脏早期损伤的诊断提供灵敏可靠的指标,从而帮助临床诊断早期肾损伤并及时干预,提高患者的治愈率。
Objective To explore the clinical value of urinary proteins analysis including urine α1-microglobulin （α1-MG）, β2-microglobulin （β2-MG）, microalbuminuria （MA）, urinary total protein （UTP） and N-acetyl-β-D-glueo-aminidase （NAG） in the diagnosis of early renal injury. Methods Eighty urine samples of hypertensive disease and diabetes with no obvious pathological glomerular, renal tubular lesions and normal urine routine examination were collected, which may be abnormal in the early stage of renal injury. Eighty healthy urine samples were collected during the same period as healthy control group. The levels of α1-MG, β2-MG, MA, UTP and NAG were detected and the results were statistically analyzed. Results When it occurred in early renal injury, for example hypertension and diabetes mellitus, urinary α1-MG, β2-MG, MA, UTP, NAG all increased in varying degrees. There were good correlations among the five test indexes and the differences were statistically significant compared against the healthy control group [α1-MG （rag/L）： 19.7±1.4 vs. 5.3 ± 1.6, β2-MG （rag/L）： 10.8±0.04 vs. 1.2±0.03, MA （mg/L）： 56.3±6.7 vs. 8.6±4.2, UTP （g/L）： 0.395±0.097 vs. 0.069±0.028, NAG （U/L）： 78.4 ± 7.6 vs. 17.9 ± 5.1, all P 〈 0.01]. Conclusions Combined detection of urinary α1-MG, β2-MG, MA, UTP, NAG can provide sensitive and reliable indicators for the diagnosis of early renal injury so as to help the clinical diagnosis of early renal injury and timely intervention to improve the recovery rate.
出处
《实用检验医师杂志》
2017年第3期159-162,共4页
Chinese Journal of Clinical Pathologist
关键词
尿蛋白分析
早期肾损伤
临床价值
Urinary proteins analysis
Early renal injury
Clinical value
