摘要
旨在探讨CaN/NFAT信号通路相关基因在鸡生长发育早期不同表型骨骼肌中的表达规律以及各基因之间的调控联系。利用荧光定量PCR技术检测隐性白羽鸡2种表型骨骼肌(腓肠肌外侧头和趾长伸肌)中CaN/NFAT信号通路相关基因(CnAα、CnB1、NFATc3、MEF2C和PPARGC1A)在出生后不同生长阶段(0、1、3、5、7和9周龄)的相对表达,并对这些基因的表达进行相关性分析。结果表明,除CnB1基因外,其他4个基因mRNA在2种表型骨骼肌中的表达趋势均较相似;2种表型骨骼肌中5个基因的表达从7周龄到9周龄均呈上升的趋势;同周龄2种表型骨骼肌相比,除腓肠肌外侧头PPARGC1A基因各周龄表达均高于趾长伸肌外,其他4个基因表达均是在3周龄时腓肠肌外侧头高于趾长伸肌,而在7和9周龄时则低于趾长伸肌;相关分析结果表明,除CnAα与NFATc3、CnAα与PPARGC1A基因的表达在2种表型肌肉中呈负相关外,其余各基因表达之间均呈正相关。提示,鸡骨骼肌CnAα、CnB1、NFATc3、MEF2C和PPARGC1A基因表达发育模式具有年龄和表型特异性,可能参与调控鸡骨骼肌肌纤维的生长和类型转换。
The objective of the present study was to investigate the expression profile of CaN/ NFAT-dependent pathway genes, and their correlation analysis in 2 phenotypically distinct skele- tal muscles were studied in chicken during the early development, qRT-PCR was used to detect the mRNA expression of CnAa, CnB1, NFATc3, MEF2C and PPARGC1A genes in lateral gas- trocnemius and extensor digitorum longus of Recessive White chicken at 0,1,3,5,7 and 9 week age, the correlation among expression of these genes was analyzed. The results showed that the expression trends of all the genes between 2 phenotypically distinct skeletal muscles were similar except CnB1, and the expression level of these genes in 2 phenotypically distinct skeletal muscles was all raised from 7 to 9 week age. Comparison among the expression of these genes in 2 phenotypically distinct skeletal muscle showed that the expression level of PPARGCIA in the lateral gastrocnemius was higher than that in the extensor digitorurn longus at each detected age, and the expression level of other 4 genes in the lateral gastrocnemius was higher at 3 week age and lower at 7 and 9 week age than that in the extensor digitorum longus. Correlation analysis among the 5 genes expression levels in the 2 phenotypically distinct skeletal muscles showed that there was a negative correlation between the CnAα and 2 genes （NFATc3 and PPARGCIA）, and positive correlations among the expression of other genes. Overall, the expression patterns of ChAco, CnB1, NFATc3, MEF2C and PPARGCIA genes in chicken skeletal muscle are age and phenotype specific, which may be related to the regulation of the myofiber growth and type transition.
作者
盛中伟
单艳菊
束婧婷
章明
姬改革
屠云洁
范建华
邹剑敏
SHENG Zhong-wei SHAN Yan-ju SHU Jing-ting ZHANG Ming JI Gai-ge TU Yun-jie FAN Jian-hua ZOU Jian-min(Jiangsu Institute of Poultry Sciences, Yangzhou 225125, China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2017年第10期1825-1832,共8页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金(31301967
31572358)
江苏省自然科学基金(BK20161322
BK20151317)
现代农业产业技术体系建设专项资金(CARS-42)
江苏省农业科技自主创新资金(CX(15)1009)
江苏省重点研发计划(现代农业)(BE2015344)
扬州市科技计划项目(YZ2014144)
