摘要
实验室条件下,观测不同剂量-时间短链氯化石蜡(SCCPs)暴露下红树蚬(Polymesoda erosa)血液及鳃组织的4种氧化逆境标志物(SOD、CAT、GST酶活性及MDA含量)的响应特征.结果表明:在低浓度(0.5,1mg/L)和中浓度(5mg/L)胁迫组,血液和鳃组织SOD、GST酶基本表现为随胁迫时间延长,酶活性逐渐上升的趋势;高浓度(10,20mg/L)胁迫组SOD、GST酶在胁迫初期表现出很高的活性,之后逐渐下降.CAT酶在胁迫初期(1d)就表现出最高的酶活性,之后酶活性逐渐降低并最终受到抑制.低浓度胁迫组MDA含量呈现上升-下降-上升的趋势;中等浓度及以上胁迫组,MDA含量持续升高.抗氧化系统在低于5mg/L的SCCPs胁迫组能够有效发挥作用,而高于此浓度的胁迫组,抗氧化系统经过初期的应急反应后,随胁迫时间延长逐渐被破坏.本文还探讨了利用红树蚬作为指示生物的可行性.
This study aimed to investigate the response characteristics of 4 oxidative stress biomarkers, the activities of superoxide dismutase（SOD）, catalase（CAT）, glutathione-S-transferase（GST） and the level of malondialdehyde（MDA） in blood and gill tissue of the Polymesoda erosa, under the condition of exposure to short-chain chlorinated paraffins（SCCPs） at different concentrations or with different exposure times. The results showed that SOD and GST activities in the blood and gill tissue increased along with the increase of exposure time under low stress（0.5mg/L, 1mg/L） or medium stress（5mg/L） of SCCPs. However, under high stress（10mg/L, 20mg/L）, the activities of SOD and GST were high at the beginning of SCCPs exposure and then decreased gradually. CAT exhibited the highest activity at the initial stage（1d） of SCCPs exposure, and then its activity decreased gradually and finally was completely inhibited. In low stress group, the level of MDA appeared an up-down-up trend, however, in medium and high stress groups, the level of MDA increased continuously with the increase of exposure time. Taken together, the antioxidant system of the Polymesoda erosa could efficiently response to SCCPs when the concentration of SCCPs was lower than 5mg/L. However, when the concentration of SCCPs was higher than 5mg/L, after an initial emergency response, the antioxidant system was destroyed with the extending of exposure time. In addition, this study also explored the feasibility of using Polymesoda erosa as a bio-indicator for environment monitoring.
出处
《中国环境科学》
EI
CAS
CSSCI
CSCD
北大核心
2017年第10期3962-3971,共10页
China Environmental Science
基金
广西自然科学基金项目(2016GXNSFBA380033)
广西科学院基本科研业务费资助(15YJ22HSL11)
关键词
红树蚬
短链氯化石蜡
氧化逆境标志物
Polymesoda erosa
short-chain chlorinated paraffins
oxidative stress biomarkers
