摘要
目的探讨针刺对阿尔茨海默病模型小鼠SAMP8的行为学影响及其作用机制。方法将60只6月龄雄性SAMP8小鼠随机分为针刺治疗组、模型对照组和非穴位对照组,每组20只,另备同月龄雄性SAMR1小鼠20只作为正常对照组。针刺治疗组采用针刺肾俞、百会、血海、膈俞进行干预。8星期后,采用Morris水迷宫对各组小鼠的行为学进行检测,同时采用western blot检测小鼠海马组织Flotillin-1、NEP以及A?42表达情况。结果 Morris水迷宫实验中,与模型对照组比较,针刺治疗组小鼠的逃避潜伏期明显缩短(P<0.05),其停留在原平台象限的时间和穿越原平台次数增加(P<0.05);与模型对照组比较,非穴位对照组小鼠的逃避潜伏期无明显差别(P>0.05),其停留在原平台象限的时间和穿越原平台次数也无明显差别(P>0.05)。Flotillin-1在针刺治疗组的表达较模型对照组明显减弱(P<0.05),而在非穴位对照组与模型对照组之间,Flotillin-1的表达水平无明显差别(P>0.05);NEP在针刺治疗组的表达较模型对照组明显增强(P<0.05),而在非穴位对照组与模型对照组之间,NEP的表达水平无明显差别(P>0.05);A?42在针刺治疗组的表达较模型对照组明显减弱(P<0.05),而在非穴位对照组与模型对照组之间,A?42的表达水平无明显差别(P>0.05)。结论针刺能够明显改善AD模型小鼠SAMP8的学习记忆能力,降低SAMP8小鼠海马Flotillin-1含量及上调NEP的表达,从而减少A?42表达,减轻神经毒性,发挥神经保护作用。
Objective To investigate the effect of acupuncture on behaviors and its mechanism of action in a SAMP8 mouse model of Alzheimer disease. Methods Sixty 6-month-old male SAMP8 mice were randomized to acupuncture, model and non--acupoint groups. Twenty male SAMP1 mice of the same age constituted a normal control group. The acupuncture group received intervention by acupuncture at Shenshu, Baihui, Xuehai and Geshu. After eight weeks, a behavioral test was performed using the Morris water maze in every group of mice. The expressions of Flotillin-1, NEP and Aβ42 in mouse hippocampus were determined by western blot. Results The Morris water maze test showed that as compared with the model group, escape latency shortened significantly （P〈0.05） and the time spent in the former platform quadrant and the number of former platform position crossings increased （P〈0.05） in the acupuncture group of mice; escape latency, the time spent in the former platform quadrant and the number of former platform position crossings had no significant differences in the non-meridian-acupoint group of mice （all P〉0.05）. As compared with the model group, Flotillin-1 expression decreased significantly in the acupuncture group （P〈0.05） but had no significant difference in the non-meridian-acupoint group （P〉0.05）; NEP expression increased significantly in the acupuncture group （P〈0.05） but had no significant difference in the non-meridian-acupoint group （P〉0.05）; Aβ42 expression decreased significantly in the acupuncture group （P〈0.05） but had no significant difference in the non-meridian-acupoint group （P〉0.05）. Conclusions Acupuncture can markedly improve learning and memory abilities in a SAMP8 mouse model of AD and reduce Flotillin-1 content and upregulate NEP expression in SAMP8 mouse hippocampus to decrease Aβ42 expression, relieve neurotoxicity and produce a neuroprotective effect.
作者
李广诚
龙聪
朱宏
董克礼
任丹
梁梅亭
李雅悦
LI Guang-chen;LONG Cong;ZHU Hong;DONG Ke-li;REN Dan;LIANG Mei-ting;LI Ya-yue(Department of Traditional Chinese Medicine, Central South University Third Xiangya Hospital,Changsha 410013,China)
出处
《上海针灸杂志》
2017年第11期1356-1360,共5页
Shanghai Journal of Acupuncture and Moxibustion
基金
国家自然科学基金青年基金项目(81202730)
湖南省自然科学基金项目(2011JJ3097)
中央高校基本科研业务经费项目(2012QNZT162)
湖南省中医药科技项目(201413)
