摘要
目的目前现代医学对于唾液腺的放射性损伤的机制仍未完全明确,治疗和防护措施也是处于探索阶段。文中探讨颌下腺放射性损伤机制,观察乌梅喷雾剂对放射性颌下腺损伤后的影响。方法 84只健康雄性Wistar大鼠按随机数字表法分为正常组、模型组、实验组,每组28只。3组均给予正常饮食外,实验组大鼠颌下腺区照射损伤后给予乌梅喷雾剂口腔喷雾干预(3次/d);模型组照射损伤后不加干预。于照射后第1、7、14、28天每组取7只大鼠称取体重、收集唾液,计算唾液流率;处死后摘取颌下腺组织应用RT-PCR及Western blot技术检测自噬相关因子Atg5的mRNA及蛋白表达水平,从分子生物学水平检测细胞自噬水平的变化。结果照射后第7天,正常组体重[(239.87±16.50)g]高于模型组[(213.84±14.42)g]和实验组[(222.71±11.14)g],差异有统计学意义(P<0.05)。照射后第14、28天,与正常组比较,模型组和实验组体重均减少(P<0.05);与模型组比较,实验组体重明显增加(P<0.05)。照射后第1、14天,模型组、实验组唾液流率低于正常组,但差异无统计学意义(P>0.05)。照射后第7天,正常组和实验组唾液流[(49.29±16.90、50.99±6.79)μL/min]率高于模型组[(30.13±13.19)μL/min],差异有统计学意义(P<0.05)。照射后第28天,正常组唾液流率[(69.29±11.32)μL/min]高于模型组[(49.26±14.13)μL/min]和实验组[(46.56±13.60)μL/min],差异有统计学意义(P<0.05)。照射后第1、7、14天,模型组和实验组Atg5的mRNA相对表达高于正常组(P<0.05)。照射后第1、7、14天,与正常组比较,模型组、实验组Atg5蛋白表达有上升趋势;照射后第1、7天,模型组与实验组间Atg5蛋白表达无明显差异。照射后第28天,与正常组相比,模型组Atg5蛋白表达下降。结论自噬活性可能与放射性颌下腺损伤发生早期有关,乌梅喷雾剂可促进抗凋亡相关因子修复颌下腺放射性损伤。
Objective At present, modern medicine cannot yet clarifythe mechanism of radiation-induceddamage （RID） to salivary glands and its treatment and protective measures remain in the exploration stage. This study was to explore the mechanism of RIDto the submandibular gland and observe the effect of Dark Plum Spray （DPS） on the submandibular gland after RID in order to provide some evidence for its further application.Methods Using the random number table, 84 healthy Wistar male rats were divided into a normal, an RID model, and an experimental group,all fed normally. The rats of the RID model groupwere left untreated, while those of the experimental group were intervened with DPS, tid, after RID. On the 1st, 7th, 14th, and 28th day after irradiation, 7 rats were taken from each group for measurement of the body weight,collection of the saliva, and calculation of the salivary flow rate. The submandibular glands were harvested for determination of the mRNA and protein expressions of autophagy-related Atg5 by RT-PCR and Western blot.Results In the 7th day after irradiation, the average body weight in NG／[（239.87±16.50）g／] was significantly higher （P〈0.05） than UG／[（213.84±14.42）g／] and EG／[（222.71±11.14）g／]. In the 14th ,28th day after irradiation, the average body weight in UG and EG were significantly lower （P〈0.05） than NG; the average body weight in EG was significantly higher （P〈0.05） than UG. In the 1st , 14th day after irradiation, the salivary flow rate in UG and EG were lower than NG but there were no significantly difference（P＞0.05）. In the 7th day after irradiation, the salivary flow rate in NG／[（49.29±16.90）μL/min／] and EG／[（50.99±6.79）μL/min／] were significantly higher （P〈0.05） than UG／[（30.13±13.19）μL/min／]. In the 28th day after irradiation, the salivary flow rate in NG／[（69.29±11.32）μL/min／] were significantly higher （P〈0.05） than UG／[（49.26±14.13）μL/min／] and EG／[（46.56±13.60）μL/min／]. In the 1st , 7th and 14th day after irradiation, RT-PCR showed that the expressions of Atg5 in UG and EG were significantly higher（P〈0.05） than NG. In the 1st , 7th and 14th day after irradiation, Western blot showed that the expressions of Atg5 in UG and EG is on the upper trend than NG; In the 1st , 7th day after irradiation, there were no significantly difference between UG and EG.In the 28th day after irradiation, Western blot showed that the expressions of Atg5 in UG is on the declining trend than NG.Conclusion The autophagic activity of submandibular gland cells may associated with early radiation-induced injury, and Dark Plum Spray may enhance the action of theanti-apoptosis cytokine in repairingradiation-induceddamageto the submandibular gland.
作者
陈倩怡
陈佩仪
李碧霞
叶景云
张嘉鹏
CHEN Qian-yi;CHEN Pei-yi;LI Bi-xia;YE Jing-yun;ZHANG Jia-peng(School of Nursing, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, Guangdong, China)
出处
《医学研究生学报》
CAS
北大核心
2017年第11期1186-1190,共5页
Journal of Medical Postgraduates
基金
广东省自然科学基金(2014A030313405)
关键词
乌梅喷雾剂
颌下腺
放射性损伤
自噬
Dark Plum Spray
Submandibular gland
Radiation-induced damage
Autophagy
