摘要
目的:探讨穴位贴敷诱发小鼠背部接触性皮炎模型制备及评价方法。方法:以经典的2,4-二硝基氟苯(DNFB)诱发的小鼠背部接触性皮炎模型作为参照,制备不同穴位贴敷方法诱发的小鼠背部接触性皮炎模型,观察皮损肉眼下及光镜下组织病理学特点。结果:肉眼下穴位贴敷造模小鼠皮损与DNFB造模小鼠皮损颜色、形态、质地等相似,符合接触性皮炎肉眼下表现。光镜下皮损组织病理学均示穴位贴敷诱发接触性皮炎模型小鼠皮损皮肤表皮角质层破坏,真皮层炎细胞浸润与DNFB诱发接触性皮炎模型小鼠表现存在高度相似性,符合接触性皮炎的病理学改变。结论:成功建立穴位贴敷诱发的小鼠背部接触性皮炎模型,以肉眼下及光镜下病理作为评价方法。
Objective： To establish and evaluate a mice model of back contact dermatitis which is induced by acupoint sticking. Methods： Compare to the classical contact dermatitis mice model which is induced by 2,4-dinitrofluorobenzene （DNFB） , we established a mice model of back contact dermatitis induced by different acupoint sticking methods. We observed the macroscopic and microscopical characteristics of skin lesions with different kinds of acupoint sticking. Results ： we found the high resemblance of the color, shape and texture between acupoint sticking models and DNFB model. Acupoint sticking models were highly in accord with DNFB model in the changes of epidermal damage and dermis inflammatory cells infiltration under the light microscope, which fitted in the changes of contact dermatitis. Conclusion：We successfully established a mice model of back contact dermatitis induced by acupoint sticking, the method was evaluated by macroscopic and microscopic pathology.
出处
《中国中医基础医学杂志》
CSCD
北大核心
2017年第10期1433-1435,共3页
JOURNAL OF BASIC CHINESE MEDICINE
基金
国家自然科学基金资助项目(81674065)-基于Th1免疫应答增强探讨穴位贴敷诱发接触性皮炎对哮喘气道炎症的反向调节机制
江苏省科技厅重点研发计划(社会发展)项目(BE2017771)-基于拉曼光谱原位监测皮炎程度的穴位贴敷技术治疗哮喘的临床研究
江苏省中医药局项目(YB2017026)-咳喘停巴布剂穴位贴敷治疗哮喘复发的中医康复临床研究
关键词
穴位贴敷
接触性皮炎模型
小鼠
Acupoint Sticking
Contact Dermatitis Model
Mice
