摘要
目的:探讨铁线莲总皂苷对佐剂性关节炎(AA)大鼠的治疗作用及其机制。方法:Wistar大鼠随机分为6组:正常对照组,模型对照组,阳性对照组(吲哚美辛,10 mg獉kg-1),铁线莲总皂苷高、中、低剂量组,每组10只。用弗氏完全佐剂建立AA大鼠模型,观察药物对大鼠体质量和足爪肿胀度的影响;测定大鼠血清中超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)、谷胱甘肽过氧化物酶(GSH-PX)的水平,以及血清中炎症因子白介素-8(IL-8)、IL-10和肿瘤坏死因子-α(TNF-α)的含量。结果:给药后第8天开始,与模型对照组相比,铁线莲总皂苷能有效缓解大鼠体质量变化,缓解大鼠的足爪肿胀度(P<0.05或P<0.01),高剂量铁线莲总皂苷与阳性对照组相比差异无统计学意义(P>0.05),低剂量组与高、中剂量组比较差异有统计学意义(P<0.05)。铁线莲总皂苷各给药组均能在一定程度上影响AA大鼠血清中SOD、MDA、NO和GSH-PX水平,除铁线莲总皂苷低剂量组对NO指标影响不显著外,其余各组与模型对照组相比差异均有统计学意义(P<0.05或P<0.01),其中MDA、NO和GSH-PX水平在高、低剂量组间差异有统计学意义(P<0.05)。与模型对照组相比,铁线莲总皂苷各组大鼠血清中IL-8和TNF-α含量显著降低,IL-10含量显著升高(P<0.05或P<0.01);高剂量组在调节IL-10和TNF-α的作用上与阳性对照组差异无统计学意义(P>0.05),其中IL-8和IL-10在高、低剂量组间差异有统计学意义(P<0.05)。结论:铁线莲总皂苷对AA具有良好的治疗作用,其机制可能与降低制脂质过氧化及抑制致炎因子有关。
Objective：To investigate the effect of total saponins from Clematis florida Thunb. on rat model of adjuvant arthritis （ AA） . Methods：Wistar rats were randomly divided into six groups：the normal control group, the model control group, the positive control group （ indomethacin, 10 mg·kg-1 ） , high, medium and low dose groups of total saponins from Clematis florida Thunb. with ten rats in each group. The rat model of AA was established with Freund＆#39;s complete adjuvant and the effects of drugs on body weight and foot swelling of AA rats were observed. After the animals were sacrificed, the serum levels of superoxide dismutase （ SOD） , mal-onaldehyde （ MDA） , nitric oxide （ NO） and glutathion peroxidase （ GSH-PX） were determined. The serum levels of interleukin-8 （ IL-8）, IL-10 and tumor necrosis factor-α（TNF-α） were detected by ELISA. Results：On the 8th day of drug administration, total sapo-nins from Clematis florida Thunb. could effectively alleviate the changes in the body weight and foot swelling of the rats to a certain ex-tent when compared with the model control group （P 〈0. 05 or P 〈0. 01）. There were no significant differences between the high dose group and the positive control group （P〉0. 05）, and there were significant differences between the low dose group and the high/medium dose groups （P〈0. 05）. The levels of SOD, MDA, NO and GSH-PX in serum of AA rats were significantly affected by the total saponins from Clematis florida Thunb. . Except for NO indicator of low dose group, the differences between the other groups and the model control group were statistically significant （P〈0. 05 or P〈0. 01）, and there were significant differences in MDA, NO and GSH-PX levels between high dose group and low dose group （P〈0. 05）. Compared with those in the model control group, the serum levels of IL-8 and TNF-αin each total saponins from Clematis florida Thunb. group significantly decreased and the content of IL-10 sig-nificantly increased （P〈0. 05 or P〈0. 01）. There was no significant differences in the levels of IL-10 and TNF-α between the high dose group and the positive control group （P〉0. 05）, and the differences in IL-8 and IL-10 between high dose group and low dose group were statistically significant （P〈0. 05）. Conclusion： The total saponins from Clematis florida Thunb. have good therapeutic effect on AA, and the underlying mechanism may be related to the reduction of lipid peroxidation and the inhibition of pro-inflammatory cytokines.
出处
《中国药师》
CAS
2017年第10期1773-1776,共4页
China Pharmacist
关键词
铁线莲总皂苷
佐剂性关节炎
抗炎
抗氧化
Total saponins from Clematis florida Thunb.
Adjuvant arthritis
Anti-inflammation
Anti-oxidation
