摘要
通过研磨辅助超声波法进行单因素和响应面实验,以优化根瘤土壤杆菌中辅酶Q_(10)的提取条件。得出的最佳工艺条件为:丙酮研磨料液比为1∶6,研磨时间为5 min;超声波破碎输出功率为260 W,工作总时间为14 min,每次辐射时间1.3 s。优化后辅酶Q_(10)的提取率可达135.82%。结果与理论值相符合,并且明显优于常规超声波法提取,因此,该方法具有一定的实际应用价值。
We studied the extraction of coenzyme Q（10） from Agrobacterium tumefaciens with grinding-ultrasonic method. Optimum working conditions were gained by single factor test and response surface experiments. The optimal conditions for coenzyme Q（10） extraction were as follows： the ratio of acetone 1∶6,total grinding time by acetone 5 min,output power of ultrasonic disruption 260 W,work time 1.3 s,total time 14 min. Under the optimized conditions,the extraction rate of coenzyme Q（10） could reach 135.82%. This method is reliable and superior to the traditional method of ultrasonic extracting,so it is of great application value.
出处
《苏州科技大学学报（自然科学版）》
CAS
2017年第4期47-53,共7页
Journal of Suzhou University of Science and Technology（Natural Science Edition）
基金
苏州市科技计划项目(SYN201317)
苏州科技学院科研基金资助项目(XKZ201411)
关键词
辅酶Q10
研磨法
超声波破碎法
响应面法
根瘤土壤杆菌
coenzyme Qlo
grinding method
ultrasonic disruption
response surface methodology
Agrobacterium tumefaciens
