摘要
目的探讨细菌培养联合靶序列富集多重聚合酶链反应(Tem-PCR)在肺炎患儿病原检测中的价值。方法选取2015年1-12月该院住院肺炎患儿141例,收集深部呼吸道引物进行细菌培养,采用Tem-PCR检测常见病原菌的特异性DNA。结果 141例患儿中83例患儿标本培养呈阳性,阳性率为58.87%(83/141);共分离出85株病原菌,病原菌检出率为60.28%(85/141);Tem-PCR检测阳性率为40.43%(57/141),病原菌检出率为50.35%(71/141)。细菌培养联合Tem-PCR检测的病原菌检出率为78.01%(110/141),明显高于细菌培养和Tem-PCR的检出率(P<0.05);其中,流感嗜血杆菌、肺炎链球菌、大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌及鲍曼不动杆菌的检出率依次为28.37%(40/141)、19.15%(27/141)、8.51%(12/141)、6.38%(9/141)、5.67%(8/141)、5.67%(8/141)、2.84%(4/141)。结论细菌培养联合Tem-PCR检测能够提高住院肺炎患儿的病原菌检出率,提高临床细菌病原学评估价值。
Objective To investigate the value of bacterial culture combined with target sequence enrichment multiplex polymerase chain reaction（Tem-PCR）in detection for the bacterial etiology in hospitalized children with pneumonia.Methods A total of141 hospitalized children with pneumonia in hospital between January to December 2015 were collected,deep respiratory aspirate sample of the children was cultured by conventional plate and special selective plate,and the aspirate samples amplified for DNA of common bacterial pathogens were tested with Tem-PCR.Results In 141 cases of children,the total positive rate by bacterial culture was 58.87%（83/141）,and the pathogen detection rate was 60.28%（85/141）;the positive rate and pathogen detection rate by TemPCR was 40.43%（57/141）and 50.35%（71/141）.The pathogen detection rate of 14 bacteria by bacterial culture combined with Tem-PCR was 78.01%（110/141）,which was higher than that of the bacterial culture and Tem-PCR.And the combined detection rates of Haemophilus influenza,Streptococcus pneumoniae,Escherichia coli,Staphylococcus aureus,Pseudomonas aeruginosa and Acinetobacter Bauman were 28.37%（40/141）,19.15%（27/141）,8.51%（12/141）,6.38%（9/141）,5.67%（8/141）and 2.84%（4/141）,respectively.Conclusion The bacterial culture combined with Tem-PCR detection could improve the positive rate of bacterial pathogens in hospitalized children with pneumonia,as well as improve the value of clinical assessment for pathogenic bacteria.
出处
《检验医学与临床》
CAS
2017年第23期3506-3508,共3页
Laboratory Medicine and Clinic
关键词
肺炎
聚合酶链反应
细菌培养
pneumonia
polymerase chain reaction
bacterial culture
