摘要
目的观察自行设计的载药纳米膜在预防新西兰大白兔胆囊切除术后腹腔粘连形成中的作用。方法40只新西兰大白兔按随机数字表法随机分为4组:空白对照组、几丁糖组、纳米膜组及载药纳米膜组,观察各组新西兰大白兔胆囊切除术后不同时间点(术后第7、14、21及28天)的腹腔粘连情况。结果 (1)空白对照组大白兔术后胆囊窝粘连严重,几丁糖组、纳米膜组和载药纳米膜组的粘连情况较轻,其中载药纳米膜组的粘连情况最轻。几丁糖组、纳米膜组及载药纳米膜组的腹腔粘连评分均明显低于空白对照组(P<0.05),载药纳米膜组的腹腔粘连评分明显低于几丁糖组(P<0.05)和纳米膜组(P<0.05),而纳米膜组与几丁糖组的腹腔粘连评分比较差异无统计学意义(P>0.05)。(2)术后第14天时,纳米膜组及载药纳米膜组的纳米膜均有降解,降解面积均较小;术后第21天时,2组的降解面积均增大;术后第28天时,2组的降解面积继续增大并且基本降解完毕。不同时间点纳米膜组及载药纳米膜组的纳米膜降解评分比较差异均无统计学意义(P>0.05)。结论本研究初步研究结果提示,载药纳米膜可从物理屏障及药物治疗两方面联合预防术后腹腔粘连,为预防普外科腹腔手术后粘连的研究以及为将来研发新型的预防腹腔粘连材料提供了新思路。
Objective To observe effect of self-designed drug-loaded nanofilm in preventing postoperative peritoneal cavity adhesion during cholecystectomy in New Zealand white rabbit. Methods The 40 New Zealand white rabbits were randomly divided into blank control group, chitosan group, nanofilm group, and drug-loaded nanofilm group using random number table, the peritoneal cavity adhesions after cholecystectomy at different time （on day 7, 14, 21, and 28） were observed among these 4 groups. Results The adhesion of gallbladder forssa was serious in the blank control group and the adhesion situation had obviously improved among the other three groups, furthermore, the adhesion of the drug-loaded nanofilm group was the slightest. The adhesion score was significantly decreased in the chitosan group, the nanofilm group, or the drug-loaded nanofilm group as compared with the blank control group （P〈0.05）, which in the drug-loaded nanofilm group was significantly decreased as compared with the chitosan group （P〈0.05） or the nanofilm group （P〈0.05）, which had no significant difference between the chitosan group and the nanofilm group （P〉0.05）. The nanofilm was degraded on day 14 after surgery and basically completely degraded on day 28 after surgery. The nanofilm degradation points had no significant differences between the nanofilm group and the drug-loaded nanofilm group at different time （P〉0.05）. Conclusions Drug-loaded nanofilm could prevent postoperative peritoneal cavity adhesion from physical barrier and drug therapy. It provides a new idea for prevention of peritoneal cavity adhesion after general surgery and research and development of new material to prevent peritoneal cavity adhesion in future.
出处
《中国普外基础与临床杂志》
CAS
2017年第12期1468-1472,共5页
Chinese Journal of Bases and Clinics In General Surgery
关键词
腹腔粘连
载药纳米膜
胆囊切除
peritoneal cavity adhesion
drug-loaded nanofilm
cholecystectomy
