摘要
菜豆金色花叶病毒属(Begomovirus)病毒是热带亚热带地区多种作物的重要病原,杂草作为该属病毒的中间寄主在病害发生流行中具有重要作用。本研究通过克隆、测序和生物信息学分析,对3株具有曲叶症状的凹头苋(Amaranthus lividus)进行菜豆金色花叶病毒属病毒的分离分析。从这些凹头苋中共分离到2种菜豆金色花叶病毒属病毒和3种beta卫星。序列分析显示,其中一种病毒与云南番茄黄化曲叶病毒相似性最高(96%),另一种与中国胜红蓟黄脉病毒相似性最高(96.5%或91%)。Beta卫星的分析显示,其中一种与云南番茄黄化曲叶beta卫星相似性最高(94.3%),另一种与赛葵曲叶beta卫星相似性最高(92%),最后一种与中国番茄曲叶beta卫星相似性最高(91%)。重组分析表明,分离物YN4331-69是一个重组病毒,是由中国胜红蓟黄脉病毒YN4326-60和一个尚未发现的菜豆金色花叶病毒属病毒重组形成。这是首次报道凹头苋被不同的菜豆金色花叶病毒属病毒及其伴随的beta卫星侵染,表明凹头苋是一个适宜该属病毒的中间寄主。
Begomoviruses are among the most damaging pathogens causing epidemics in crops in many tropical and subtropical regions. As intermediate hosts of begomoviruses, weeds play crucial role in the emergence of plant virus disease epidemics. In this work, three Amaranthus lividus plants showing leaf curl symptoms were sampled followed by cloning, sequencing and bioinformatic analysis. Two distinct monopartite begomoviruses and three distinct betasatellites were identified in these Amaranthus lividus. Sequence analysis showed that one begomovirus shared the highest identity （96%） with Tomato yellow leaf curl Yunnan virus（TYLCYnV）and the other shared 96.5% or 91% with Ageratum yellow vein China virus（AYVCNV）. One of the betasatellites shared the highest identity （94.3%） with Tomato yellow leaf curl Yunnan betasatellite （TYLCYnB）, and the rest shared 92% with Malvastrum leaf curl betasatellite （MaLCB） and 91% with Tomato leaf curl China betasatellite （ToLCCNB）, respectively. Recombination analysis showed that the YN4331 69 isolate was generated by the recombination between AYVCNV YN4326 60 and an unidentified begomovirus. This is the first report of mixed infections by begomoviruses associated with betasatellites in Amaranthus lividusand indicated that Amaranthus lividusis an appropriate intermediate host for the virus in the genus Begomovirus.
出处
《植物病理学报》
CAS
CSCD
北大核心
2017年第6期738-746,共9页
Acta Phytopathologica Sinica
基金
国家自然科学基金云南联合基金资助项目(U1136606)
国家自然科学基金资助项目(31260421)
