摘要
目的:建立高效液相色谱法-电喷雾串联质谱联用分析大花罗布麻中植物激素的分析方法.方法:采用C_(18)SPE柱提取大花罗布麻叶中植物激素,在色谱柱为Agilent SB-C_(18)(50 mm×2.1 mm,1.8μm),0.5%甲酸水-甲醇为流动相,在MRM模式下,ESI负离子模式,毛细管电压为4 k V,干燥器流量为10 L·min^(-1),干燥器温度为350℃等条件下,建立液相色谱法-电喷雾串联质谱(LC-MS)联用分析大花罗布麻叶中五种植物激素的方法.结果:大花罗布麻叶中五种植物激素ABA、IBA、IAA、GA_3、JA含量分别为9.800×10~3μg·kg^(-1)、3.073×10~4μg·kg^(-1)、1.403×10~4μg·kg^(-1)、4.475×10~4μg·kg^(-1)、2.508×10~4μg·kg^(-1);五种激素在各自对应的线性范围内线性关系良好.结论:方法灵敏、准确,适合用于大花罗布麻叶中植物激素含量的测定.
Objective：To develop a LC-MS method for the determination of plant hormones in Poacyzuan hendersonii. Methods： The plant hormones were isolated and purified by C18 SPE chromatographic techniques. Chromatographic separation was per- formed on an Agilent SB-Cms （50 mm × 2. 1 mm, 1.8 μm）. The MS system was operated in the multiple reaction monitoring modes. The MS detection parameters were as follows ： the capillary voltage was 4 kV, capillary temperature was 350 ℃, and sheath gas flow rate was 10 L · min1. Results：The content of five kinds of plant hormones in the leaves of P. hendersonii were 9. 800 ×10^3 μg·kg^-1, 3. 073 × 10^4 μg·kg^-1 , 1. 403× 10^4 μg·kg^-1 , 4. 475× 10^4 μg·kg^-1 , 2. 508 × 10^4 μg·kg^-1 , respectively. Conclusion： The method is highly sensitive and selective, suitable for the analysis of plant hormones in P. hender-
出处
《西南民族大学学报（自然科学版）》
CAS
2017年第6期593-598,共6页
Journal of Southwest Minzu University(Natural Science Edition)
基金
西南民族大学研究生创新型科研项目(CX2017SZ086)
