摘要
目的 :分离培养人骨髓间充质干细胞 ,进行超微结构观察。方法 :冲洗出流产胎儿股骨骨髓内容物 ,梯度离心 ,取含有骨髓间充质干细胞的低密度血小板层进行培养 ,分别在透射电镜、扫描电镜下进行超微结构的全面观察。结果 :透射电镜下可见细胞有两种不同的形态结构 ,细胞间有许多缝隙连接 ;扫描电镜下可见细胞表面有短而粗的微绒毛突起。结论
Objective:To culture mesenchymal stem cells (MSCs) derived from bone marrow of human aborted fetal and observe their ultrastructures. Methods:Rinse marrow from aborted fetal femur to a sterile tube with MSCBM medium. The tube was spun to pellet the cells, and then the cell pellets were separated by gradient centrifugation. The lowest density cells including MSCs were cultured in MSCBM medium contained 10% fetal bovine serum. The ultrastructures of the cultured cells were observed by transmission electron microscopy and scanning electron microscopy. Results:Easy handling methods to isolate and culture MSCs were developed in the study. Two kinds of MSCs including active MSCs and inactive MSCs could be identified and some gap junctions between two adjacent cells were seen under transmission electron microscope. Many dumpy microvilli were on the surface of the cell. Conclusion: In vitro cultured MSCs have the structural base of being seed cells in the field of tissue engineering.
出处
《电子显微学报》
CAS
CSCD
北大核心
2002年第4期373-376,共4页
Journal of Chinese Electron Microscopy Society
基金
国家重点基础研究发展规划 (973 )(G19990 5 40 3 8)
国家自然科学基金资助项目(No .3 0 0 70 3 68)
广州市科委科技攻关项目 (2 0 0 0 Z 0 17 0 1 0 5 )
