摘要
目的探讨miR-199a-3p对卵巢癌SK0V3细胞增殖、侵袭能力的影响及其作用机制。方法以卵巢癌SK0V3细胞系为研究对象,将进入对数生长期的细胞按照生长情况分为miR-199a-3p过表达组、miR-199a-3p抑制组和对照组,观察miR-199a-3p过表达和抑制效率及其对卵巢癌SK0V3细胞增殖、侵袭及c-Met、MMP-2和CD44蛋白表达的影响。结果与对照组比较,miR-199a-3p过表达组SK0V3细胞中miR-199a-3p呈高表达状态,且SK0V3细胞与细胞克隆数量、侵袭细胞数量、c-Met及其下游蛋白MMP-2和CD44的表达水平均降低,而miR-199a-3p抑制组SK0V3细胞中miR-199a-3p表达下降,SK0V3细胞与细胞克隆数量、侵袭细胞数量、c-Met及其下游蛋白MMP-2和CD44的表达水平均显著增加,差异均有统计学意义(P<0.05)。结论 miR-199a-3p可能通过抑制c-Met、MMP-2和CD44蛋白的表达进而影响卵巢癌SK0V3细胞的侵袭和迁移,可能成为治疗卵巢癌的新方法。
Objective To investigate effects of miR-199a-3p in proliferation and invasion of SK0V3 cells in ovarial cancer and its mechanisms. Methods SK0V3 cell line of ovarial cancer were recruited in this study, and SK0V3 cells in log-arithmic growth phase were divided into miR-199a-3p overexpression, miR-199a-3p inhibition group and control groups. The miR-199a-3p overexpression and inhibition rates, and its effects on proliferation and invasion of SK0V3 cells and Met, MMP-2 and CD44 protein expressions were observed. Results Compared with those in control group, in miR-199a-3p overexpression group, miR-199a-3p in SK0V3 cancer cells was over expressed, and numbers of SK0V3 cells, cell clone formation and inva-sion cells, expressions of c-Met, MMP-2 and CD44 of c-Met downstream proteins were significantly decreased; in miR-199a-3p inhibition group, miR-199a-3p expression in SK0V3 cancer cells was decreased, and numbers of SK0V3 cells, cell clone formation and invasion cells,protein expressions of c-Met, MMP-2 and CD44 of c-Met downstream proteins were sig-nificantly increased （P〈0. 05）. Conclusion Thet miR-199a-3p can affect invasion and transference of SK0V3 cells in ovar-ial cancer by inhibiting c-Met, MMP-2 and CD44 protein expressions, and therefore miR-199a-3p may be used as a potential target in treatment of ovarian cancer.
作者
张丽娟
张旭力
王娟
王芳
ZHANG Li-juan;ZHANG Xu-li;WANG Juan;WANG Fang(Department of Obstetrics and Gynecology,Maternal andChild Health Hospital of Wuxue,Wuxue,Hubei 435400,China;Department of Pharmacy,the First People＇ s Hospital ofWuxue,Wuxue,Hubei 435400,China;Department of Obstetrics Tumor,Cancer Hospital of Hubei Province,Wuhan430079,China;Molecular Biology Center,Medical College of Wuhan University,Wuhan 430079,China)
出处
《临床误诊误治》
2017年第12期90-93,共4页
Clinical Misdiagnosis & Mistherapy
基金
湖北省卫生厅医学科研项目(QJX2010-401)
