摘要
目的对米糠蜡进行传统致畸试验和微核试验检测,对其致畸和致突变作用进行评价。方法对小鼠进行急性经口毒性试验,记录中毒症状及死亡情况;进行微核试验,染色观察其嗜多染红细胞中微核发生率;对大鼠进行致畸试验并计算其畸形率。结果急性毒性试验:米糠蜡对雌雄小鼠经口LD50均>15g/kg bw。小鼠骨髓细胞微核试验:各剂量组微核率与溶剂对照组比较,差异均无统计学意义(P>0.05),而阳性对照组微核率与溶剂对照组及各剂量组比较,差异有统计学意义(雌性:χ~2=96.75,P<0.05;雄性:χ~2=149.21,P<0.05)。大鼠致畸试验:孕鼠与胎鼠的生长发育各剂量组与阴性对照组比较,差异均无统计学意义(P>0.05),胎鼠的畸形率各剂量组与阴性对照组比较,差异均无统计学意义(P>0.05),而阳性对照组胎鼠畸形率(72.91%)高于阴性对照组(6.85%)及各剂量组(6.56%、8.20%、8.70%),差异均有统计学意义(χ~2=4.12,P=0.036;χ~2=17.34,P<0.05)。结论米糠蜡对大鼠无致畸作用,对小鼠无致突变作用。
Objective To experimentally observe the teratogenic and mutagenic effects of rice bran wax in mice and rats. Methods Mice and rates in experimental group were given rice bran wax by oral gavage and those in control group received only vehicle.The traditional teratogenicity test and micronucleus test were used by observing the symptoms of poisoning and death of mice and the formation of micronuclei in erythrocytes in bone marrow of rats. Results Oral MTD of rice bran wax was greater than 15 g/kg bw in both female and male mice in acute toxicity test.In micronucleus test,the micronucleus formation rates of bone marrow cell in each dose group were similar with that of vehicle control group（P〉0.05）,but were significantly different compared with the positive control group（P〈0.05）.Meanwhile,the differences of murine growth and development indicators and deformity rates of embryo in each dose group were not significant compared with the vehicle control group（P〉0.05）.However,the malformation rate in viscera and skeletal of rats in positive group was significantly higher than that of rats in control group as well as those in each dose group（χ^2=4.12,P=0.036;χ^2=17.34,P〈0.05）. Conclusions The teratogenic effect and mutagenic effect of rice bran wax were not obvious and need to be further determined.
作者
李学敏
田若涛
李建国
张颖
李淑琴
边林秀
赵海峰
LI Xue-min;TIAN Ruo-tao;LI Jian-guo;ZHANG Ying;LI Shu-qin;BIAN Lin-xiu;ZHAO Hai-feng(Shanxi Center for Disease Control and Prevention, Taiyuan, Shanxi 030012, China)
出处
《中国预防医学杂志》
CAS
CSCD
2017年第11期823-826,共4页
Chinese Preventive Medicine
基金
山西省实验动物专项资金项目2013(K13)
关键词
米糠蜡
急性毒性试验
致畸试验
微核试验
致突变试验
小鼠
大鼠
Rice bran wax
Acute toxicity test
Teratogenicity test
Micronucleus test
Mutagenicity test
Mice
Rats
