摘要
目的研究溃疡性结肠炎(UC)患者血清吲哚胺2,3-双加氧酶(IDO)水平与调节性T细胞(Treg)的关系。方法选择2015年1月至2017年2月海南省人民医院治疗的UC患者70例进行研究,将其作为观察组。另选同期在院内进行体检的健康志愿者70例作为对照组,对比两组血清IDO和Treg的表达水平,观察组不同疾病时期及活动期UC不同病情程度患者血清IDO和Treg的表达水平,分析UC患者血清IDO水平与Treg表达水平的相关性。结果观察组血清IDO水平明显较对照组升高,而Treg的表达水平较对照组降低,差异有统计学意义(P<0.05)。观察组活动期UC患者的血清IDO明显较缓解期升高,而Treg的表达水平较缓解期降低,差异有统计学意义(P<0.05)。活动期UC患者不同病情程度之间的血清IDO和Treg的表达水平比较,差异有统计学意义(P<0.05)。中度组和重度组血清IDO水平较轻度组升高,而Treg的表达水平较轻度组明显降低,差异有统计学意义(P<0.05)。重度组患者的血清IDO水平较中度组明显升高,而Treg的表达水平较中度组明显降低,差异有统计学意义(P<0.05)。Spearman秩相关分析显示,UC患者血清IDO水平与Treg表达水平之间呈负相关(r=-0.798,P=0.000)。结论 UC患者血清IDO水平与Treg的表达水平呈负相关,共同影响UC的发生与发展。
Objective To study the relationship between serum indoleamine 2 ,3-dioxygenase（IDO） and the level of regulatory T cells（Treg） in the patients with ulcerative colitis（UC）. Methods Seventy cases of UC treated in the Hainan Provincial Peopled Hospital from January 2015 to February 2017 were selected as the observation group. In the same period, 70 healthy volunteers undergoing physical examination were selected as the control group. The levels of serum IDO and Treg were compared between the two groups？ the expression levels of serum IDO and Treg in of the patients with different disease activity periods and different severity de-grees of UC were observed,and the correlation between the level of serum IDO and the Treg expression level in the patients with UC was analyzed. Results The serum IDO level in the observation group was significantly increased compared with that in the control group,while the Treg expression level decreased which compared with the control group,the difference was statistically significant （P〈0. 05）. The serum IDO level in the pa-tients with activity period of the observation group was significantly increased compared with that in the pa-tients with remission period,but the Treg expression level was decreased compared with that in the patients with remission period,the difference was significant （P〈0. 05）. The serum IDO and Treg expression levels had statistical difference among different disease degrees in the patients with active UC （P〈0. 05）. The serum IDO level in the moderate and severe groups increased which compared with that in the mild group,while the Treg expression level was significantly decreased compared with that in the mild group,the difference was sig-nificant （P 〈0. 05）. The level of serum IDO in the severe group was significantly higher than that in the mod-erate group ？ while the Treg expression level was significantly lower than that in the moderate group, the difference was statistically significant （P 〈C 0. 05）. Conducting the correlation analysis according to the Spearman method found that there was a negative correlation between the serum IDO level and the Treg ex-pression level in the patients with UC（r= -0. 798 ,P = 0. 000）. Conclusion The level of serum IDO in the pa-tients with UC is negatively correlated with the Treg expression level, which jointly influence the occurrence and development of UC.
出处
《国际检验医学杂志》
CAS
2018年第1期21-24,共4页
International Journal of Laboratory Medicine
基金
国家自然科学基金资助项目(81360603)
