摘要
目的优选丹参祛痘涂膜剂提取工艺条件和制备方法,并对其抗炎作用进行观察。方法采用L_9(3)~4正交实验法考察温度、溶剂浓度、提取时间及提取次数对隐丹参酮和丹参酮ⅡA提取率的影响,优选醇提条件;正交实验优选出涂膜剂的基质组方;采用二甲苯所致小鼠耳廓肿胀模型研究该涂膜剂的抗炎作用。结果醇提工艺条件为8倍量90%乙醇75℃回流提取3次;时间分别为2、1、1 h,制备涂膜剂的基质最优组方为聚乙烯醇(PVA)∶甘油∶西黄蓍胶=1.0 g∶2.5 mL∶1.0 g;与基质对照组比较,祛痘涂膜剂中剂量(1%)与高剂量(2%)具有抗炎作用,能显著降低小鼠耳廓肿胀度(P<0.05)。结论丹参祛痘涂膜剂提取与制备工艺稳定可控,成膜性好,无刺激性,无毒,具有较显著的的抗炎作用。
Objective To optimize the extraction and to tetermine the anti-inflammation effect. Methods preparation of acne-removing Danshen plastics and L9 （3）4 orthogonal test was applied to explore the effect of temperature, solvent concentration, extraction time and extraction times on the extraction rate of cryptotanshinone and tanshinone Ⅱ A. The matrix formula of the plastics was optimized by the orthogonal test. The anti-inflammation effects were studied by determining the ear swelling degree and inhibition rate of mice ear swelling model induced by xylene. Results The optimal condition for extraction was as follows： heating reflux with 8 times of 90% ethanol at 75 ℃ for 3 times, extraction time was 2 h, 1 h and 1 h, respectively. The best matrix formula of the plastics was PVA： glycerin： tragacanth = 1.0 g ： 2.5 mL ： 1.0 g. Compared with the matrix control group, the medium dose （1%） and high dose （2%） acne-removing Danshen plastics reduced the ear swelling degree in mice remarkably （P 〈 0.05）. Conclusion The extraction and preparation of acne-removing Danshen plastics are stable and reproducible, with easy coating, non-irritating, non-toxic, and significant anti-inflammation effect.
出处
《中南药学》
CAS
2017年第12期1704-1708,共5页
Central South Pharmacy
基金
2013年云南省大学生创新创业训练计划项目(No.201310680002)
云南省科技厅应用基础研究项目(No.2014FZ089)
关键词
丹参祛痘涂膜剂
醇提工艺
涂膜剂制备
抗炎作用
acne-removing Danshen plastics
alcohol extraction process
preparation of plastics
anti- inflammatory
