摘要
基于辣椒全基因组数据信息,利用生物信息学方法对CaGRAS基因家族进行了系统鉴定和进化分析,并通过Real-time PCR方法检测了CaGRAS家族组织表达模式和对PEG6000、盐胁迫的应答。结果表明:在辣椒中存在54个CaGRAS基因,除11号染色体外其余染色体均有分布,外显子数1~3,等电点4.97~9.13;系统进化分析显示CaGRAS基因可分为8个进化群;CaGRAS基因具有不同的表达模式,在根、茎、叶片和茎尖中优势表达的基因分别有34、8、3和8个;多数CaGRAS基因能响应PEG6000和盐胁迫,其中CaGRAS11、CaGRAS30、CaGRAS40、CaGRAS44和CaGRAS50受到PEG6000和盐胁迫的强烈诱导。本研究为深入解析CaGRAS家族基因的功能奠定了一定基础。
In this study,we systematically investigated the member of CaGRAS gene family in pepper（Capsicum annuum L.）and elucidated their phylogenetic relationships,tissue-specific transcription patterns,and responses to PEG6000 and salt stresses. A total of 54 CaGRAS genes were identified and they distributed on all chromosomes except the 11 th. The number of exons ranged from 1 to 3,and isoelectric points ranged from 4.97 to 9.13. Phylogenetic analysis showed that CaGRAS genes were clustered into eight phylogenetic groups. The CaGRAS genes exhibited different expression patterns in distinct pepper tissues. Among them,34 genes highly expressed in roots,eight genes in stems,three genes in mature leaves and eight genes in shoot tips. Moreover,most CaGRAS genes were responsive to PEG6000 and salt stresses. Some genes including CaGRAS11,CaGRAS30,CaGRAS40,CaGRAS44 and CaGRAS50 were greatly induced by both PEG6000 and salt stresses. These results will provide a foundation for further functional research on CaGRAS genes in pepper.
出处
《园艺学报》
CAS
CSCD
北大核心
2017年第12期2305-2317,共13页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31172001)
国家现代农业产业技术体系建设专项资金项目(CARS-25)
公益性行业(农业)科研专项(201303014)
中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS)
关键词
辣椒
GRAS基因家族
进化分析
表达特性
pepper
GRAS gene family
phylogeny analysis
expression characteristic
