摘要
目的：建立半夏中β-谷甾醇的含量测定方法,比较半夏3种不同炮制品清半夏、法半夏、姜半夏中β-谷甾醇的含量差异。方法：利用紫外可见分光光度法建立,优选测定波长、显色试剂用量、显色时间及显色温度,建立β-谷甾醇的含量测定方法;测定半夏3种不同炮制品中β-谷甾醇的含量。结果：确定了β-谷甾醇显色的条件为检测波长为420 nm,加浓H2SO41.0 mL,并于50℃水浴显色4 min,建立β-谷甾醇的含量测定方法,其线性回归方程为y=0.010 1x＋0.026 1,R2=0.999 3,β-谷甾醇在1070μg/mL浓度范围内与吸光度呈良好线性关系。清半夏、法半夏、姜半夏3种半夏炮制品中β-谷甾醇的含量分别为1.780、1.172、0.844 mg/g。结论：半夏3种炮制品中β-谷甾醇含量清半夏最高,法半夏其次,姜半夏最低。结果可为半夏炮制品的深入研究,尤其是临床上选用半夏组方提供了较好的实验参考依据。
Objective： To establish a method for the determination of β-Sitosterol and compare the content of β-Sitosterol in three kinds of different processed products of Pinellia ternata. Methods： By using ultraviolet-visible spectrophotometry,we established a method for the determination of β-Sitosterol and optimized the measuring wavelength,eagent amount,color time and temperature. Then we determinated the content of β-Sitosterol in Qing Banxia,Fa Banxia and Jiang Banxia. Results：The method for the determination of β-Sitosterol： wavelength 420 nm,mixed H2SO4 1. 0 mL,color 4 minutes in 50 ℃ water bath; standard curve regression equation： y = 0. 010 1 x ＋ 0. 026 1,R2= 0. 999 3. There was a good linear relationship in 10 70 μg/mL. The contents of β-Sitosterol were 1. 780 mg/g,1. 172 mg/g,0. 844 mg/g respectively in Qing Banxia,Fa Banxia and Jiang Banxia. Conclusion： The content of β-Sitosterol in Qing Banxia is the highest,in Fa Banxia is the second,and that in Jiang Banxia is the minimum. The results could be helpful for the further research of the processed products of Pinellia ternata,providing good experimental references for clinical selection of prescription which containing Pinellia ternata.
出处
《中华中医药学刊》
CAS
北大核心
2018年第1期42-44,共3页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金项目(81473587)
浙江省自然科学基金项目(LR16H270001)
关键词
半夏
炮制品
紫外可见分光光度法
Β-谷甾醇
Pinellia ternata
different proceed products
ultraviolet-visible spectrophotometry
β-Sitosterol
